Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.16 extracted from

  • Li, Y.; Roux, C.; Lazereg, S.; LeCaer, J.P.; Laprevote, O.; Badet, B.; Badet-Denisot, M.A.
    Identification of a novel serine phosphorylation site in human glutamine:fructose-6-phosphate amidotransferase isoform 1 (2007), Biochemistry, 46, 13163-13169.
    View publication on PubMed
Search for more literature for 2.6.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cell and Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
S243E increase in activity, 2fold lower Km value for D-fructose 6-phosphate than wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 D-fructose 6-phosphate mutant S243E, 37°C, pH 7.5 Homo sapiens
1.04
-
 D-fructose 6-phosphate wild-type, 37°C, pH 7.5 Homo sapiens
1.8
-
 L-gamma-glutamyl-p-nitroanilide both wild-type and mutant S243E, 37°C, pH 7.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 glutamine:fructose-6-phosphate amidotransferase isoform 1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein enzyme is phosphorylated in vivo, phosphorylation site is residue S243. Phosphorylation at Ser243 stimulates glucosamine 6-phosphate synthesizing activity, lowers amidohydrolyzing activity in the absence of fructose 6-phosphate, and lowers Km of fructose 6-phosphate 2fold, but has no effect on UDP-GlcNAc inhibition. Phosphorylation is mediated by AMP-activated protein kinase and calcium/calmodulin-dependent kinase II in vitro Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 50 mM Tris-HCl, pH 7.8, 200 mM NaCl, 1 mM fructose 6-phosphate, 2 mM tris(2-carboxyethyl)phosphine hydrochloride, 200 mM imidazole, 10% glycerol, stable for up to 12 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-gamma-glutamyl-p-nitroanilide + H2O determination of amidohydrolysing activity Homo sapiens L-glutamine + p-nitroaniline
-
 ?
L-glutamine + D-fructose 6-phosphate
-
 Homo sapiens L-glutamate + D-glucosamine 6-phosphate
-
 ?