Literature summary for 2.6.1.16 extracted from

Chmara, H.; Z�hner, H.
The inactivation of glucosamine synthetase from bacteria by anticapsin, the C-terminal epoxyamino acid of the antibiotic tetaine (1984), Biochim. Biophys. Acta, 787, 45-52.

Inhibitors

Inhibitors	Comment	Organism
1,1'-dithiodiformamidine	irreversible inhibition	Bacillus thuringiensis
1,1'-dithiodiformamidine	irreversible inhibition	Escherichia coli
1,1'-dithiodiformamidine	irreversible inhibition	Paenarthrobacter aurescens
1,1'-dithiodiformamidine	irreversible inhibition	Pseudomonas aeruginosa
5,5'-dithionitrobenzoic acid	irreversible inhibition	Bacillus thuringiensis
5,5'-dithionitrobenzoic acid	irreversible inhibition	Escherichia coli
5,5'-dithionitrobenzoic acid	irreversible inhibition	Paenarthrobacter aurescens
5,5'-dithionitrobenzoic acid	irreversible inhibition	Pseudomonas aeruginosa
6,6'-Dithiodinicotinic acid	irreversible inhibition	Bacillus thuringiensis
6,6'-Dithiodinicotinic acid	irreversible inhibition	Escherichia coli
6,6'-Dithiodinicotinic acid	irreversible inhibition	Paenarthrobacter aurescens
6,6'-Dithiodinicotinic acid	irreversible inhibition	Pseudomonas aeruginosa
anticapsin	L-glutamine protects, irreversible inhibition	Bacillus thuringiensis
anticapsin	L-glutamine protects, irreversible inhibition	Escherichia coli
anticapsin	L-glutamine protects, irreversible inhibition	Paenarthrobacter aurescens
anticapsin	L-glutamine protects, irreversible inhibition	Pseudomonas aeruginosa
iodoacetamide	irreversible inhibition	Bacillus thuringiensis
iodoacetamide	irreversible inhibition	Escherichia coli
iodoacetamide	irreversible inhibition	Paenarthrobacter aurescens
iodoacetamide	irreversible inhibition	Pseudomonas aeruginosa
N-ethylmaleimide	irreversible inhibition	Bacillus thuringiensis
N-ethylmaleimide	irreversible inhibition	Escherichia coli
N-ethylmaleimide	irreversible inhibition	Paenarthrobacter aurescens
N-ethylmaleimide	irreversible inhibition	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.225	-	L-glutamine	pH 7.5, 37°C	Paenarthrobacter aurescens
0.4	-	L-glutamine	pH 7.5, 37°C	Escherichia coli
0.6	-	L-glutamine	pH 7.5, 37°C	Bacillus thuringiensis
0.85	-	L-glutamine	pH 7.5, 37°C	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Bacillus thuringiensis	-	-	-
Escherichia coli	-	-	-
Paenarthrobacter aurescens	-	-	-
Pseudomonas aeruginosa	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.2	-	-	Pseudomonas aeruginosa
3.6	-	-	Bacillus thuringiensis
4	-	-	Escherichia coli
8	-	-	Paenarthrobacter aurescens

Storage Stability

Storage Stability	Organism
5°C, potassium phosphate buffer, pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, 0.5 mM L-glutamine, 0.05 mM D-glucose 6-phosphate, 600 mM sucrose, stable for few weeks	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-glutamine + D-fructose 6-phosphate	-	Escherichia coli	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Pseudomonas aeruginosa	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Paenarthrobacter aurescens	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Bacillus thuringiensis	L-glutamate + D-glucosamine 6-phosphate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.00011	-	anticapsin	pH 7.5, 37°C	Pseudomonas aeruginosa
0.00053	-	anticapsin	pH 7.5, 37°C	Paenarthrobacter aurescens
0.00056	-	anticapsin	pH 7.5, 37°C	Escherichia coli
0.00075	-	anticapsin	pH 7.5, 37°C	Bacillus thuringiensis