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Literature summary for 2.6.1.14 extracted from

  • Maul, D.M.; Schuster, S.M.
    Kinetic properties and characteristics of mouse liver mitochondrial asparagine aminotransferase (1986), Arch. Biochem. Biophys., 251, 585-593.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Mus musculus
Aminooxyacetate
-
Mus musculus
D-cycloserine weak Mus musculus
glycine asparagine/pyruvate reaction Mus musculus
HgCl2
-
Mus musculus
homoserine asparagine/pyruvate reaction Mus musculus
hydroxylamine
-
Mus musculus
KCN
-
Mus musculus
L-aspartate weak, asparagine/pyruvate reaction Mus musculus
L-cysteine
-
Mus musculus
leucine asparagine/pyruvate reaction Mus musculus
L-glutamate weak, asparagine/pyruvate reaction Mus musculus
L-glutamine asparagine/pyruvate reaction Mus musculus
methionine asparagine/pyruvate reaction Mus musculus
additional information strong substrate and product inhibition Mus musculus
N-ethylmaleimide
-
Mus musculus
(NH4)2SO4 at high concentration Mus musculus
norvaline asparagine/pyruvate reaction Mus musculus
p-chloromercuribenzoate
-
Mus musculus
phenylalanine
-
Mus musculus
serine asparagine/pyruvate reaction Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
L-methionine cosubstrate phenylpyruvate Mus musculus
1
-
L-asparagine cosubstrate phenylpyruvate Mus musculus
0.43
-
L-glutamine cosubstrate phenylpyruvate Mus musculus
1.4
-
L-homoserine cosubstrate phenylpyruvate Mus musculus
6.8
-
L-methionine cosubstrate 2-oxosuccinamate Mus musculus
4.8
-
L-alanine cosubstrate 2-oxosuccinamate Mus musculus
6.5
-
L-norvaline cosubstrate 2-oxosuccinamate Mus musculus
28.7
-
L-leucine cosubstrate 2-oxosuccinamate Mus musculus
24.7
-
L-homocysteine cosubstrate 2-oxosuccinamate Mus musculus
21.4
-
L-glutamine cosubstrate 2-oxosuccinamate Mus musculus
28.8
-
L-2-aminobutanoic acid cosubstrate 2-oxosuccinamate Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Mus musculus
-
-
cytosol
-
Mus musculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70800
-
mouse mitochondrial enzyme Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
highest activity at pH 8.5 in N,N-bis(2-hydroxyethyl)glycine buffer and 4-(2-hydroxyethyl)-1-piperazinepropane sulfonic acid buffer, low activity in borate buffer and glycylglycine buffer Mus musculus
5.08
-
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + glyoxylate
-
Mus musculus 2-oxosuccinamate + glycine
-
?
2-oxosuccinamic acid + L-alanine
-
Mus musculus L-asparagine + pyruvate
-
?
2-oxosuccinamic acid + L-2-aminobutanoic acid
-
Mus musculus L-asparagine + 2-oxobutanoate
-
?
2-oxosuccinamic acid + L-homocysteine
-
Mus musculus L-asparagine + 4-mercapto-2-oxobutanoate
-
?
2-oxosuccinamic acid + L-norvaline
-
Mus musculus L-asparagine + 2-oxopentanoate
-
?
2-oxosuccinamic acid + L-leucine
-
Mus musculus L-asparagine + 4-methyl-2-oxopentanoate
-
?
2-oxosuccinamic acid + L-methionine
-
Mus musculus L-asparagine + 4-methylsulfanyl-2-oxobutanoate
-
r
2-oxosuccinamic acid + L-glutamine
-
Mus musculus L-asparagine + L-glutamate
-
?
L-asparagine + pyruvate
-
Mus musculus 2-oxosuccinamate + L-alanine
-
?
L-asparagine + 4-hydroxy-2-oxoglutarate
-
Mus musculus 2-oxosuccinamate + 2-amino-4-hydroxyglutarate
-
?
L-asparagine + 2-oxoisohexanoate
-
Mus musculus 2-oxosuccinamate + 2-aminoisohexanoate
-
?
L-asparagine + 4-methylsulfanyl-2-oxobutanoate weak Mus musculus 2-oxosuccinamate + L-methionine
-
r
phenylpyruvate + L-homoserine
-
Mus musculus phenylalanine + 4-hydroxy-2-oxobutanoate
-
?
phenylpyruvate + L-glutamine
-
Mus musculus L-phenylalanine + 2-oxoglutaramate
-
?
phenylpyruvate + methionine
-
Mus musculus L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate
-
?
phenylpyruvate + L-asparagine
-
Mus musculus L-phenylalanine + 2-oxosuccinamate
-
?