BRENDA - Enzyme Database
show all sequences of 2.6.1.14

Asparagine transaminase from rat liver

Cooper, A.J.L.; J. Biol. Chem. 252, 2032-2038 (1977) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
glycine
-
Rattus norvegicus
homoserine
asparagine/pyruvate reaction; DL-isomer
Rattus norvegicus
L-2-aminobutanoate
weak, asparagine/pyruvate reaction
Rattus norvegicus
L-alanine
weak, asparagine/pyruvate reaction
Rattus norvegicus
L-aspartate
-
Rattus norvegicus
leucine
asparagine/pyruvate reaction; weak
Rattus norvegicus
L-glutamine
-
Rattus norvegicus
L-methionine-SR-sulfoxime
asparagine/pyruvate reaction
Rattus norvegicus
methionine
asparagine/pyruvate reaction; L-isomer
Rattus norvegicus
serine
asparagine/pyruvate reaction
Rattus norvegicus
S-methyl-L-cysteine
asparagine/pyruvate reaction
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
100
-
2-oxobutanoate
cosubstrate L-asparagine
Rattus norvegicus
0.45
-
phenylpyruvate
cosubstrate L-asparagine
Rattus norvegicus
2.5
-
2-oxosuccinamate
cosubstrate L-asparagine
Rattus norvegicus
0.35
-
4-methylsulfanyl-2-oxobutanoate
cosubstrate L-asparagine
Rattus norvegicus
5.1
-
pyruvate
cosubstrate L-asparagine
Rattus norvegicus
0.077
-
glyoxylate
cosubstrate L-asparagine
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glyoxylate + amino acid
Rattus norvegicus
-
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
Reaction ID
L-asparagine + a 2-oxo carboxylate = 2-oxosuccinamate + an amino acid
ping-pong mechanism
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
similar activity in glycylglycine, ammediol and Tris/HCl buffer
Rattus norvegicus
0.25
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-oxosuccinamic acid + aspartate
-
637253
Rattus norvegicus
L-asparagine + 2-oxo-propane-1,3-dioate
-
-
-
?
2-oxosuccinamic acid + L-2-aminobutanoic acid
-
637253
Rattus norvegicus
L-asparagine + 2-oxobutanoate
-
-
-
r
2-oxosuccinamic acid + L-alanine
-
637253
Rattus norvegicus
L-asparagine + pyruvate
-
-
-
?
2-oxosuccinamic acid + L-cysteine
-
637253
Rattus norvegicus
L-asparagine + 3-mercapto-2-oxopropanoate
-
-
-
?
2-oxosuccinamic acid + L-glutamine
-
637253
Rattus norvegicus
L-asparagine + L-glutamate
-
-
-
?
2-oxosuccinamic acid + L-homoserine
-
637253
Rattus norvegicus
L-asparagine + 4-hydroxy-2-oxobutanoate
-
-
-
?
2-oxosuccinamic acid + L-leucine
-
637253
Rattus norvegicus
L-asparagine + 4-methyl-2-oxopentanoate
-
-
-
?
2-oxosuccinamic acid + L-methionine
-
637253
Rattus norvegicus
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
-
-
-
r
2-oxosuccinamic acid + L-methylcysteine
-
637253
Rattus norvegicus
L-asparagine + ?
-
-
-
?
2-oxosuccinamic acid + L-phenylalanine
-
637253
Rattus norvegicus
L-asparagine + 2-oxo-3-phenylpropanoate
-
-
-
?
2-oxosuccinamic acid + L-serine
-
637253
Rattus norvegicus
L-asparagine + 3-hydroxy-2-oxopropanoate
-
-
-
?
glyoxylate + amino acid
-
637253
Rattus norvegicus
?
-
-
-
?
glyoxylate + L-asparagine
-
637253
Rattus norvegicus
? + 2-oxosuccinamate
-
-
-
?
L-asparagine + 2-oxobutanoate
weak
637253
Rattus norvegicus
2-oxosuccinamate + 2-aminobutanoate
-
-
-
r
L-asparagine + 2-oxosuccinamate
-
637253
Rattus norvegicus
2-oxosuccinamate + L-asparagine
-
-
-
?
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
-
637253
Rattus norvegicus
2-oxosuccinamate + L-methionine
-
-
-
r
L-asparagine + glyoxylate
-
637253
Rattus norvegicus
2-oxosuccinamate + glycine
-
-
-
?
L-asparagine + pyruvate
-
637253
Rattus norvegicus
2-oxosuccinamate + L-alanine
-
-
-
?
additional information
the following 2-oxo acids are inactive at concentrations of 20 mM: 2-oxoglutarate, oxaloacetate, 2-oxoisopentanoate, L-2-oxo-3-methylvalerate, 3-sulfopyruvate, 3-hydroxy-2-oxobutanoate, trimethylpyruvate
637253
Rattus norvegicus
?
-
-
-
?
phenylpyruvate + L-asparagine
-
637253
Rattus norvegicus
L-phenylalanine + 2-oxosuccinamate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
8.6
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glycine
-
Rattus norvegicus
L-aspartate
-
Rattus norvegicus
L-glutamine
-
Rattus norvegicus
leucine
weak
Rattus norvegicus
methionine
L-isomer
Rattus norvegicus
L-alanine
weak, asparagine/pyruvate reaction
Rattus norvegicus
L-2-aminobutanoate
weak, asparagine/pyruvate reaction
Rattus norvegicus
leucine
asparagine/pyruvate reaction
Rattus norvegicus
methionine
asparagine/pyruvate reaction
Rattus norvegicus
S-methyl-L-cysteine
asparagine/pyruvate reaction
Rattus norvegicus
serine
asparagine/pyruvate reaction
Rattus norvegicus
homoserine
asparagine/pyruvate reaction
Rattus norvegicus
L-methionine-SR-sulfoxime
asparagine/pyruvate reaction
Rattus norvegicus
homoserine
DL-isomer
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
100
-
2-oxobutanoate
cosubstrate L-asparagine
Rattus norvegicus
0.45
-
phenylpyruvate
cosubstrate L-asparagine
Rattus norvegicus
2.5
-
2-oxosuccinamate
cosubstrate L-asparagine
Rattus norvegicus
0.35
-
4-methylsulfanyl-2-oxobutanoate
cosubstrate L-asparagine
Rattus norvegicus
5.1
-
pyruvate
cosubstrate L-asparagine
Rattus norvegicus
0.077
-
glyoxylate
cosubstrate L-asparagine
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glyoxylate + amino acid
Rattus norvegicus
-
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
similar activity in glycylglycine, ammediol and Tris/HCl buffer
Rattus norvegicus
0.25
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-oxosuccinamic acid + aspartate
-
637253
Rattus norvegicus
L-asparagine + 2-oxo-propane-1,3-dioate
-
-
-
?
2-oxosuccinamic acid + L-2-aminobutanoic acid
-
637253
Rattus norvegicus
L-asparagine + 2-oxobutanoate
-
-
-
r
2-oxosuccinamic acid + L-alanine
-
637253
Rattus norvegicus
L-asparagine + pyruvate
-
-
-
?
2-oxosuccinamic acid + L-cysteine
-
637253
Rattus norvegicus
L-asparagine + 3-mercapto-2-oxopropanoate
-
-
-
?
2-oxosuccinamic acid + L-glutamine
-
637253
Rattus norvegicus
L-asparagine + L-glutamate
-
-
-
?
2-oxosuccinamic acid + L-homoserine
-
637253
Rattus norvegicus
L-asparagine + 4-hydroxy-2-oxobutanoate
-
-
-
?
2-oxosuccinamic acid + L-leucine
-
637253
Rattus norvegicus
L-asparagine + 4-methyl-2-oxopentanoate
-
-
-
?
2-oxosuccinamic acid + L-methionine
-
637253
Rattus norvegicus
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
-
-
-
r
2-oxosuccinamic acid + L-methylcysteine
-
637253
Rattus norvegicus
L-asparagine + ?
-
-
-
?
2-oxosuccinamic acid + L-phenylalanine
-
637253
Rattus norvegicus
L-asparagine + 2-oxo-3-phenylpropanoate
-
-
-
?
2-oxosuccinamic acid + L-serine
-
637253
Rattus norvegicus
L-asparagine + 3-hydroxy-2-oxopropanoate
-
-
-
?
glyoxylate + amino acid
-
637253
Rattus norvegicus
?
-
-
-
?
glyoxylate + L-asparagine
-
637253
Rattus norvegicus
? + 2-oxosuccinamate
-
-
-
?
L-asparagine + 2-oxobutanoate
weak
637253
Rattus norvegicus
2-oxosuccinamate + 2-aminobutanoate
-
-
-
r
L-asparagine + 2-oxosuccinamate
-
637253
Rattus norvegicus
2-oxosuccinamate + L-asparagine
-
-
-
?
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
-
637253
Rattus norvegicus
2-oxosuccinamate + L-methionine
-
-
-
r
L-asparagine + glyoxylate
-
637253
Rattus norvegicus
2-oxosuccinamate + glycine
-
-
-
?
L-asparagine + pyruvate
-
637253
Rattus norvegicus
2-oxosuccinamate + L-alanine
-
-
-
?
additional information
the following 2-oxo acids are inactive at concentrations of 20 mM: 2-oxoglutarate, oxaloacetate, 2-oxoisopentanoate, L-2-oxo-3-methylvalerate, 3-sulfopyruvate, 3-hydroxy-2-oxobutanoate, trimethylpyruvate
637253
Rattus norvegicus
?
-
-
-
?
phenylpyruvate + L-asparagine
-
637253
Rattus norvegicus
L-phenylalanine + 2-oxosuccinamate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
8.6
-
Rattus norvegicus
Other publictions for EC 2.6.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
759257
Liepman
Crystal structure Of photores ...
Arabidopsis thaliana
Front. Plant Sci.
10
1229
2019
-
-
-
1
1
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
-
-
3
2
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
759546
Moriya
Identification of enzyme gene ...
Streptococcus mutans serotype c, Streptococcus mutans serotype c ATCC 700610, Streptococcus mutans serotype c UA159
J. Chem. Inf. Model.
56
510-516
2016
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
637255
hongo
Identity of rat liver mitochon ...
Rattus norvegicus
Enzyme
36
232-238
1987
-
-
-
-
-
-
1
-
3
-
-
-
-
4
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637254
Maul
Kinetic properties and charact ...
Mus musculus
Arch. Biochem. Biophys.
251
585-593
1986
-
-
-
-
-
-
21
11
5
-
1
-
-
4
-
-
1
-
-
3
2
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
21
-
11
5
-
1
-
-
-
-
1
-
3
2
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637253
Cooper
Asparagine transaminase from r ...
Rattus norvegicus
J. Biol. Chem.
252
2032-2038
1977
-
-
-
-
-
-
11
6
-
-
-
1
-
1
-
-
1
1
-
1
2
-
20
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
6
-
-
-
1
-
-
-
1
-
1
2
-
20
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637252
Meister
Enzymatic formation of L-aspar ...
Rattus norvegicus
J. Biol. Chem.
210
37-43
1954
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
14
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-