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Literature summary for 2.6.1.13 extracted from

  • Juncosa, J.I.; Lee, H.; Silverman, R.B.
    Two continuous coupled assays for ornithine-delta-aminotransferase (2013), Anal. Biochem., 440, 145-149.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.13

Application

Application Comment Organism
analysis development of two new continuous, coupled assays for ornithine-delta-aminotransferase (OAT) that are more sensitive than previous methods, measure activity in real time, and can be carried out in multi-well plates for convenience and high throughput. The first assay is based on the reduction of DELTA1-pyrroline-5-carboxylate (P5C), generated from ornithine by OAT, using human pyrroline 5-carboxylate reductase 1, which results in the concomitant oxidation of NADH to NAD+. This procedure is three times more sensitive than previous methods, and is suitable for the study of small molecules as inhibitors or inactivators of OAT or as a method to determine OAT activity in unknown samples. The second method involves the detection of L-glutamate, produced during the regeneration of the cofactor PLP of OAT by an unamplified modification of the commercially available Amplex® Red L-glutamate detection kit (Life Technologies). This assay is recommended for the determination of the substrate activity of small molecules against OAT Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.97
-
 L-ornithine assay based on detection of L-glutamate, pH 8.0, 37°C Homo sapiens
10.5
-
 L-ornithine assay based on reduction of DELTA1-pyrroline-5-carboxylate, pH 8.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P04181
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.01
-
 assay based on detection of L-glutamate, pH 8.0, 37°C Homo sapiens
5.22
-
 assay based on reduction of DELTA1-pyrroline-5-carboxylate, pH 8.0, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + 2-oxoglutarate
-
 Homo sapiens DELTA1-pyrroline-5-carboxylate + glutamate
-
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