BRENDA - Enzyme Database
show all sequences of 2.6.1.111

A novel acyl-CoA beta-transaminase characterized from a metagenome

Perret, A.; Lechaplais, C.; Tricot, S.; Perchat, N.; Vergne, C.; Pelle, C.; Bastard, K.; Kreimeyer, A.; Vallenet, D.; Zaparucha, A.; Weissenbach, J.; Salanoubat, M.; PLoS ONE 6, e22918 (2011) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0043
-
(3S)-3-aminobutanoyl-CoA
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
2.9
-
2-oxoglutarate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
20.1
-
pyruvate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
93000
-
gel fitlration
Candidatus Cloacimonas acidaminovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
Candidatus Cloacimonas acidaminovorans
-
acetoacetyl-CoA + L-glutamate
-
-
?
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
Candidatus Cloacimonas acidaminovorans Evry
-
acetoacetyl-CoA + L-glutamate
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
Candidatus Cloacimonas acidaminovorans
-
acetoacetyl-CoA + L-alanine
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
Candidatus Cloacimonas acidaminovorans Evry
-
acetoacetyl-CoA + L-alanine
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Candidatus Cloacimonas acidaminovorans
B0VH76
-
-
Candidatus Cloacimonas acidaminovorans Evry
B0VH76
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
-
741266
Candidatus Cloacimonas acidaminovorans
acetoacetyl-CoA + L-glutamate
-
-
-
?
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
-
741266
Candidatus Cloacimonas acidaminovorans Evry
acetoacetyl-CoA + L-glutamate
-
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
-
741266
Candidatus Cloacimonas acidaminovorans
acetoacetyl-CoA + L-alanine
-
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
-
741266
Candidatus Cloacimonas acidaminovorans Evry
acetoacetyl-CoA + L-alanine
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-3-phenylpropanethioate + 2-oxoglutarate
i.e. beta-phenylalanine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans
S-[2-(acetylamino)ethyl] 3-oxo-3-phenylpropanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-3-phenylpropanethioate + 2-oxoglutarate
i.e. beta-phenylalanine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans Evry
S-[2-(acetylamino)ethyl] 3-oxo-3-phenylpropanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-5-methylhexanethioate + 2-oxoglutarate
i.e. beta-homoleucine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans
S-[2-(acetylamino)ethyl] 3-oxo-5-methylhexanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-5-methylhexanethioate + 2-oxoglutarate
i.e. beta-homoleucine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans Evry
S-[2-(acetylamino)ethyl] 3-oxo-5-methylhexanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-aminobutanethioate + 2-oxoglutarate
i.e. 3-aminobutanoyl-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans
S-[2-(acetylamino)ethyl] 3-oxobutanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-aminobutanethioate + 2-oxoglutarate
i.e. 3-aminobutanoyl-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans Evry
S-[2-(acetylamino)ethyl] 3-oxobutanethioate + L-glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 46481, calculated
Candidatus Cloacimonas acidaminovorans
Synonyms
Synonyms
Commentary
Organism
3-aminobutyryl-CoA aminotransferase
-
Candidatus Cloacimonas acidaminovorans
acyl-CoA beta-transaminase
-
Candidatus Cloacimonas acidaminovorans
KAT
-
Candidatus Cloacimonas acidaminovorans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Candidatus Cloacimonas acidaminovorans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
33
-
complete loss of activity above
Candidatus Cloacimonas acidaminovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.4
-
pyruvate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
1.8
-
2-oxoglutarate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
1.8
-
(3S)-3-aminobutanoyl-CoA
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Candidatus Cloacimonas acidaminovorans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
-
inactive below
Candidatus Cloacimonas acidaminovorans
7.5
8.5
narrow
Candidatus Cloacimonas acidaminovorans
10
-
20% of maximum activity
Candidatus Cloacimonas acidaminovorans
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Candidatus Cloacimonas acidaminovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Candidatus Cloacimonas acidaminovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0043
-
(3S)-3-aminobutanoyl-CoA
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
2.9
-
2-oxoglutarate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
20.1
-
pyruvate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
93000
-
gel fitlration
Candidatus Cloacimonas acidaminovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
Candidatus Cloacimonas acidaminovorans
-
acetoacetyl-CoA + L-glutamate
-
-
?
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
Candidatus Cloacimonas acidaminovorans Evry
-
acetoacetyl-CoA + L-glutamate
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
Candidatus Cloacimonas acidaminovorans
-
acetoacetyl-CoA + L-alanine
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
Candidatus Cloacimonas acidaminovorans Evry
-
acetoacetyl-CoA + L-alanine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
-
741266
Candidatus Cloacimonas acidaminovorans
acetoacetyl-CoA + L-glutamate
-
-
-
?
(3S)-3-aminobutanoyl-CoA + 2-oxoglutarate
-
741266
Candidatus Cloacimonas acidaminovorans Evry
acetoacetyl-CoA + L-glutamate
-
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
-
741266
Candidatus Cloacimonas acidaminovorans
acetoacetyl-CoA + L-alanine
-
-
-
?
(3S)-3-aminobutanoyl-CoA + pyruvate
-
741266
Candidatus Cloacimonas acidaminovorans Evry
acetoacetyl-CoA + L-alanine
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-3-phenylpropanethioate + 2-oxoglutarate
i.e. beta-phenylalanine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans
S-[2-(acetylamino)ethyl] 3-oxo-3-phenylpropanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-3-phenylpropanethioate + 2-oxoglutarate
i.e. beta-phenylalanine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans Evry
S-[2-(acetylamino)ethyl] 3-oxo-3-phenylpropanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-5-methylhexanethioate + 2-oxoglutarate
i.e. beta-homoleucine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans
S-[2-(acetylamino)ethyl] 3-oxo-5-methylhexanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-amino-5-methylhexanethioate + 2-oxoglutarate
i.e. beta-homoleucine-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans Evry
S-[2-(acetylamino)ethyl] 3-oxo-5-methylhexanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-aminobutanethioate + 2-oxoglutarate
i.e. 3-aminobutanoyl-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans
S-[2-(acetylamino)ethyl] 3-oxobutanethioate + L-glutamate
-
-
-
?
S-[2-(acetylamino)ethyl] 3-aminobutanethioate + 2-oxoglutarate
i.e. 3-aminobutanoyl-S-(N-acetylcysteamine), N-acetylcysteamine can serve as a CoA mimic for Kat
741266
Candidatus Cloacimonas acidaminovorans Evry
S-[2-(acetylamino)ethyl] 3-oxobutanethioate + L-glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 46481, calculated
Candidatus Cloacimonas acidaminovorans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Candidatus Cloacimonas acidaminovorans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
33
-
complete loss of activity above
Candidatus Cloacimonas acidaminovorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.4
-
pyruvate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
1.8
-
2-oxoglutarate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
1.8
-
(3S)-3-aminobutanoyl-CoA
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Candidatus Cloacimonas acidaminovorans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
-
inactive below
Candidatus Cloacimonas acidaminovorans
7.5
8.5
narrow
Candidatus Cloacimonas acidaminovorans
10
-
20% of maximum activity
Candidatus Cloacimonas acidaminovorans
General Information
General Information
Commentary
Organism
physiological function
gene product Kat may participate in an alternative pathway of lysine fermentation
Candidatus Cloacimonas acidaminovorans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
gene product Kat may participate in an alternative pathway of lysine fermentation
Candidatus Cloacimonas acidaminovorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.02
-
pyruvate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
0.62
-
2-oxoglutarate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
420
-
(3S)-3-aminobutanoyl-CoA
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.02
-
pyruvate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
0.62
-
2-oxoglutarate
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
420
-
(3S)-3-aminobutanoyl-CoA
pH 8.1, 28°C
Candidatus Cloacimonas acidaminovorans
Other publictions for EC 2.6.1.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741266
Perret
A novel acyl-CoA beta-transami ...
Candidatus Cloacimonas acidaminovorans, Candidatus Cloacimonas acidaminovorans Evry
PLoS ONE
6
e22918
2011
-
-
-
-
-
-
-
3
-
-
1
4
-
5
-
-
-
-
-
-
-
-
10
1
3
1
1
-
3
1
3
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
4
-
-
-
-
-
-
-
-
10
1
1
1
-
3
1
3
-
-
-
1
1
-
3
3