BRENDA - Enzyme Database
show all sequences of 2.6.1.103

Biosynthesis of the di-meta-hydroxyphenylglycine constituent of the vancomycin-group antibiotic chloroeremomycin

Sandercock, A.M.; Charles, E.H.; Scaife, W.; Kirkpatrick, P.N.; O’Brien, S.W.; Papageorgiou, E.A.; Spencer, J.B.; Williams, D.H.; Chem. Commun. (Camb. ) 2001, 1252-12653 (2001)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression of the N-terminal His6-tagged protein in Escherichia coli
Amycolatopsis orientalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49800
-
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis A82846
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Amycolatopsis orientalis
-
-
-
Amycolatopsis orientalis A82846
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Amycolatopsis orientalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis A82846
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis A82846
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Synonyms
Synonyms
Commentary
Organism
HpgT
-
Amycolatopsis orientalis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Amycolatopsis orientalis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
a pyridoxal-phosphate protein
Amycolatopsis orientalis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of the N-terminal His6-tagged protein in Escherichia coli
Amycolatopsis orientalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
a pyridoxal-phosphate protein
Amycolatopsis orientalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49800
-
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
Amycolatopsis orientalis A82846
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Amycolatopsis orientalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin
722024
Amycolatopsis orientalis A82846
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
(S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate
-
722024
Amycolatopsis orientalis A82846
(3,5-dihydroxyphenyl)(oxo)acetate + L-glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 49800, electrospray ionisation mass spectrometry
Amycolatopsis orientalis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Amycolatopsis orientalis
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine of the glycopeptide antibiotic chloroeremomycin
Amycolatopsis orientalis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of (S)-3,5-dihydroxyphenylglycine of the glycopeptide antibiotic chloroeremomycin
Amycolatopsis orientalis
Other publictions for EC 2.6.1.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722024
Sandercock
-
Biosynthesis of the di-meta-hy ...
Amycolatopsis orientalis, Amycolatopsis orientalis A82846
Chem. Commun. (Camb. )
2001
1252-12653
2001
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