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Literature summary for 2.6.1.102 extracted from

  • Cook, P.D.; Kubiak, R.L.; Toomey, D.P.; Holden, H.M.
    Two site-directed mutations are required for the conversion of a sugar dehydratase into an aminotransferase (2009), Biochemistry, 48, 5246-5253.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.102

Protein Variants

Protein Variants Comment Organism
K186H single mutant enzyme has no detectable catalytic activity with its sugar substrate Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 GDP-4-dehydro-6-deoxy-alpha-D-mannose pH 7.5, 22°C Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate Caulobacter vibrioides
-
 GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate Caulobacter vibrioides CB15
-
 GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides
-
-
-
Caulobacter vibrioides CB15
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate
-
 Caulobacter vibrioides GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate
-
 Caulobacter vibrioides CB15 GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?

Synonyms

Synonyms Comment Organism
GDP-perosamine synthase
-
 Caulobacter vibrioides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 GDP-4-dehydro-6-deoxy-alpha-D-mannose pH 7.5, 22°C Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate pyridoxal 5'-phosphate-dependent enzyme Caulobacter vibrioides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
208
-
 GDP-4-dehydro-6-deoxy-alpha-D-mannose pH 7.5, 22°C Caulobacter vibrioides