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Literature summary for 2.6.1.102 extracted from
- Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro (2007), Biochem. Biophys. Res. Commun., 363, 525-530.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21 (DE3) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
GDP-4-dehydro-6-deoxy-D-mannose | pH and temperature not specified in the publication | Escherichia coli |  |
| 2 | - |
L-glutamate | pH and temperature not specified in the publication | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 431000 | - |
recombinant His-tagged Per fusion protein | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | Escherichia coli | - |
GDP-D-perosamine + 2-oxoglutarate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | - |
Escherichia coli | GDP-D-perosamine + 2-oxoglutarate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | recombinant His-tagged Per fusion protein | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 52 | - |
L-glutamate | pH and temperature not specified in the publication | Escherichia coli | |
| 210 | - |
GDP-4-dehydro-6-deoxy-D-mannose | pH and temperature not specified in the publication | Escherichia coli | |
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