Literature summary for 2.6.1.1 extracted from

Gharib, G.; Chohan, S.; Rashid, N.; Akhtar, M.
Heterologous gene expression and characterization of recombinant aspartate aminotransferase from Geobacillus thermopakistaniensis (2020), Protein Expr. Purif., 175, 105709 .

Search for more literature for 2.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells	Geobacillus sp. MAS1

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the enzyme is not influenced by EDTA	Geobacillus sp. MAS1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5	-	L-aspartate	at pH 8.0 and 60°C	Geobacillus sp. MAS1
2.5	-	2-oxoglutarate	using L-aspartate as a cosubstrate, at pH 8.0 and 60°C	Geobacillus sp. MAS1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the enzyme is independent from metal ions	Geobacillus sp. MAS1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	gel filtration	Geobacillus sp. MAS1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	Geobacillus sp. MAS1	-	oxaloacetate + L-glutamate	-	r

Organism

Organism	UniProt	Comment	Textmining
Geobacillus sp. MAS1	A0A6F8T0V6	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap QFF column chromatography, HiTrap SPFF column chromatography and Superdex 200X gel filtration	Geobacillus sp. MAS1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
11	-	crude extract, at pH 8.0 and 60°C	Geobacillus sp. MAS1
104	-	after 9.5fold purification, at pH 8.0 and 60°C	Geobacillus sp. MAS1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	-	Geobacillus sp. MAS1	oxaloacetate + L-glutamate	-	r
L-aspartate + 2-oxoglutarate	100% activity	Geobacillus sp. MAS1	oxaloacetate + L-glutamate	-	r
L-proline + 2-oxoglutarate	16% activity compared to L-aspartate	Geobacillus sp. MAS1	?	-	?
L-tyrosine + 2-oxoglutarate	27% activity compared to L-aspartate	Geobacillus sp. MAS1	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 42295, MALDI-TOF mass spectrometry	Geobacillus sp. MAS1
homodimer	2 * 42562, calculated from amino acid sequence	Geobacillus sp. MAS1

Synonyms

Synonyms	Comment	Organism
aspartate aminotransferase	-	Geobacillus sp. MAS1
AST	-	Geobacillus sp. MAS1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Geobacillus sp. MAS1

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	75	more than 60% activity between 50 and 75°C	Geobacillus sp. MAS1

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	75	there is no significant change in activity after 16 h incubation at 65°C. At 75°C the stability is reduced drastically and the half-life of the enzyme is reduced to 15 min	Geobacillus sp. MAS1

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
36.83	-	2-oxoglutarate	using L-aspartate as a cosubstrate, at pH 8.0 and 60°C	Geobacillus sp. MAS1
4170	-	L-aspartate	at pH 8.0 and 60°C	Geobacillus sp. MAS1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Geobacillus sp. MAS1

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.5	more than 50% activity at pH 6.0-8.5	Geobacillus sp. MAS1

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Geobacillus sp. MAS1

pI Value

Organism	Comment	pI Value Maximum	pI Value
Geobacillus sp. MAS1	calculated from amino acid sequence	-	5.5

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Release 2023.1 (January 2023)