Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Star cells | Schizosaccharomyces pombe |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 20% (w/v) polyethyleneglycol 8000 and 0.1 M sodium HEPES (pH 8.5) | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Schizosaccharomyces pombe | - |
oxaloacetate + L-glutamate | - |
r | |
L-aspartate + 2-oxoglutarate | Schizosaccharomyces pombe 972 | - |
oxaloacetate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O42652 | - |
- |
Schizosaccharomyces pombe 972 | O42652 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephacryl S300 gel filtration | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Schizosaccharomyces pombe | oxaloacetate + L-glutamate | - |
r | |
L-aspartate + 2-oxoglutarate | - |
Schizosaccharomyces pombe 972 | oxaloacetate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
AST | - |
Schizosaccharomyces pombe |
L-aspartate aminotransferase | - |
Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Schizosaccharomyces pombe |