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Literature summary for 2.6.1.1 extracted from

  • Donini, S.; Ferrari, M.; Fedeli, C.; Faini, M.; Lamberto, I.; Marletta, A.S.; Mellini, L.; Panini, M.; Percudani, R.; Pollegioni, L.; Caldinelli, L.; Petrucco, S.; Peracchi, A.
    Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism (2009), Biochem. J., 422, 265-272.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as His6-tagged protein in Escherichia coli cells. The production of the protein corresponding to the gene GOT1L1 encounters several problems: upon induction, multiple overexpressed protein species are detected in the cell lysate, and none of them could be purified by metal-affinity chromatography Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NHS2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Homo sapiens oxaloacetate + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
GOT1L1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at, coupled assay with malate dehydrogenase Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at, coupled assay with malate dehydrogenase Homo sapiens