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Literature summary for 2.6.1.1 extracted from

  • Kato, Y.; Asano, Y.; Makar, T.K.; Cooper, A.J.
    Irreversible inactivation of aspartate aminotransferase by 2-oxoglutaconic acid and its dimethyl ester (1996), J. Biochem., 120, 531-539.
    View publication on PubMed
Search for more literature for 2.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutaconate injection into mice leads to inhibition of the kidney enzyme Mus musculus
2-oxoglutaconate 2-oxoglutarate protects against inhibition, L-glutamate enhances the inhibitory effect; 30% inhibition at 25°C, pH 7.2, 10 mM, after 2 h; cytosolic and mitochondrial isozymes Rattus norvegicus
2-oxoglutaconate 2-oxoglutarate protects against inhibition, L-glutamate enhances the inhibitory effect; 30% inhibition at 25°C, pH 7.2, 10 mM, after 2 h; binds the active site of the pyridoxal 5'-phosphate enzyme form, cytosolic isozyme Sus scrofa
2-oxoglutaconic acid dimethyl ester 15% inhibition at 25°C, pH 7.0, 10 mM, after 2 h; pyridoxal 5'-phosphate enzyme form, cytosolic isozyme Sus scrofa
N-ethylmaleimide alkylation of cysteine residues Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isoenzyme Rattus norvegicus 5829
-
cytosol cytosolic isoenzyme Sus scrofa 5829
-
mitochondrion mitochondrial isoenzyme Rattus norvegicus 5739
-
mitochondrion mitochondrial isoenzyme Sus scrofa 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Mus musculus
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Rattus norvegicus
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Sus scrofa
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Rattus norvegicus
-
 male Wistar rats
-
Rattus norvegicus
-
 cytosolic and mitochondrial isozyme
-
Sus scrofa
-
 purified cytosolic isozyme
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Sus scrofa
-
kidney
-
 Mus musculus
-
kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Mus musculus oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Rattus norvegicus oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Sus scrofa oxaloacetate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
AspAT
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Mus musculus
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Rattus norvegicus
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Sus scrofa
pyridoxal 5'-phosphate bound to the active site Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
 2-oxoglutaconate cytosolic isozyme, 25°C, pH 7.2 Sus scrofa