Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Maleate | 78% inhibition of cytosolic isozyme at 4 mM, competitive to 2-oxoglutarate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
2.06 | - |
2-oxoglutarate | cytosolic isozyme, pH 6.8, 37°C | Homo sapiens | |
22.5 | - |
L-aspartate | cytosolic isozyme, pH 6.8, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isoenzyme | Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Homo sapiens | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
cytosolic isozyme | - |
Purification (Comment) | Organism |
---|---|
partial, 404fold, cytosolic isozyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, partially purified cytosolic isozyme, stable for 1 month, gradual decrease of activity afterwards | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Homo sapiens | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Homo sapiens | oxaloacetate + L-glutamate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
cytosolic isozyme | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.2 | cytosolic isozyme | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.72 | - |
Maleate | cytosolic isozyme, pH 6.8, 37°C | Homo sapiens |