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Literature summary for 2.6.1.1 extracted from

  • Vessal, M.; Taher, M.
    Partial purification and kinetic properties of human placental cytosolic aspartate transaminase (1995), Comp. Biochem. Physiol. B, 110, 431-437.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Maleate 78% inhibition of cytosolic isozyme at 4 mM, competitive to 2-oxoglutarate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Homo sapiens
2.06
-
2-oxoglutarate cytosolic isozyme, pH 6.8, 37°C Homo sapiens
22.5
-
L-aspartate cytosolic isozyme, pH 6.8, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isoenzyme Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Homo sapiens
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
cytosolic isozyme
-

Purification (Commentary)

Purification (Comment) Organism
partial, 404fold, cytosolic isozyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
4°C, partially purified cytosolic isozyme, stable for 1 month, gradual decrease of activity afterwards Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Homo sapiens oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate
-
Homo sapiens oxaloacetate + L-glutamate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
cytosolic isozyme Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.4 7.2 cytosolic isozyme Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.72
-
Maleate cytosolic isozyme, pH 6.8, 37°C Homo sapiens