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Literature summary for 2.6.1.1 extracted from
- Enzymes of nitrogen metabolism in legume nodules: partial purification and properties of the aspartate aminotransferases from lupine nodules (1981), Arch. Biochem. Biophys., 209, 524-533.
General Stability
| General Stability | Organism |
|---|---|
| complete loss of activity after freezing and thawing | Lupinus angustifolius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | product and substrate inhibition | Lupinus angustifolius |  |
| L-glutamate | product inhibition, noncompetitive to 2-oxoglutarate and competitive against aspartate | Lupinus angustifolius | |
| oxaloacetate | isozymes AAT-P1 and AAT-P2; substrate inhibition | Lupinus angustifolius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
oxaloacetate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
| 0.1 | - |
oxaloacetate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius | |
| 0.2 | - |
2-oxoglutarate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
| 0.26 | - |
2-oxoglutarate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius | |
| 2.2 | - |
L-aspartate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius | |
| 2.6 | - |
L-aspartate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
| 12 | - |
L-glutamate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
| 22 | - |
L-glutamate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Lupinus angustifolius | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 96000 | - |
isozyme AAT-P2, gel filtration | Lupinus angustifolius |
| 105000 | - |
gel filtration, isozyme AAT-P2 | Lupinus angustifolius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + 2-oxoglutarate | Lupinus angustifolius | - |
oxaloacetate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus angustifolius | - |
2 cytosolic isozymes: AAT-P1 and AAT-P2 | - |
| Lupinus angustifolius | - |
cv. Uniharvest | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes: AAT-P1 and AAT-P2, partial | Lupinus angustifolius |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Lupinus angustifolius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root nodule | - |
Lupinus angustifolius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 105 | - |
partially purified enzyme | Lupinus angustifolius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + 2-oxoglutarate | - |
Lupinus angustifolius | oxaloacetate + L-glutamate | - |
? | |
| L-aspartate + 2-oxoglutarate | absolute specificity for aspartate and 2-oxoglutarate and for glutamate and oxaloacetate | Lupinus angustifolius | oxaloacetate + L-glutamate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | AAT-P2 und AAT-P2 | Lupinus angustifolius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | - |
Lupinus angustifolius |
| 8 | 8.9 | - |
Lupinus angustifolius |
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