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Literature summary for 2.6.1.1 extracted from

  • Ryan, E.; Bodley, F.; Fottrell, P.F.
    Purification and characterization of aspartate aminotransferases from soybean root nodules and Rhizobium japonicum (1972), Phytochemistry, 11, 957-963.
No PubMed abstract available
Search for more literature for 2.6.1.1

General Stability

General Stability Organism
labile enzyme is stabilized by pyridoxyl 5'-phosphate and L-aspartate Bradyrhizobium japonicum

Inhibitors

Inhibitors Comment Organism Structure
CN- cytosolic isoform, at 5 mM Glycine max
isonicotinic acid hydrazine complete inhibition at 0.3 mM, partially reversible by pyridoxyl 5'-phosphate Bradyrhizobium japonicum
isonicotinic acid hydrazine cytosolic isozyme, only after 24 h incubation, 1 mM Glycine max
L-Cycloserine cytosolic isozyme, weak inhibition only after 24 h incubation, 1 mM Glycine max
additional information cytosolic isozyme, no inhibition by L-cysteine and EDTA Glycine max
p-chloromercuribenzoate complete inhibition at 0.15 mM Bradyrhizobium japonicum
p-chloromercuribenzoate cytosolic isozyme, 50% inhibition after 10 min at 30°C, 1 mM Glycine max
Sodium mersalyl cytosolic isozyme, 40% inhibition after 60 min at 5 mM and 30°C Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bradyrhizobium japonicum
additional information
-
 additional information
-
 Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bradyrhizobium japonicum 5829
-
cytosol 2 isozymes Glycine max 5829
-
mitochondrion mitochondrial isoenzyme Glycine max 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information cytosolic isozyme, no metal ion requirement Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Bradyrhizobium japonicum
100000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Bradyrhizobium japonicum
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Glycine max
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Bradyrhizobium japonicum 392
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
-
-
Bradyrhizobium japonicum 392
-
-
-
Glycine max
-
 3 isozymes: 2 cytosolic, 1 mitochondrial
-

Purification (Commentary)

Purification (Comment) Organism
1 cytosolic isozyme, partial, 120fold Glycine max
partial, 92fold Bradyrhizobium japonicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bradyrhizobium japonicum
additional information
-
-
 Glycine max
0.576
-
 partially purified cytosolic isozyme Glycine max
69.4
-
 partially purified enzyme Bradyrhizobium japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Bradyrhizobium japonicum oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Glycine max oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Bradyrhizobium japonicum 392 oxaloacetate + L-glutamate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 cytosolic isozyme Glycine max
8
-
-
 Bradyrhizobium japonicum

pH Range

pH Minimum pH Maximum Comment Organism
6.3 9 about 60% of activity maximum at pH 6.3 and pH 9.0 Glycine max
7 9 pH 7.0: about 45% of activity maximum, pH 9.0: about 40% of activity maximum Bradyrhizobium japonicum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Bradyrhizobium japonicum