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Literature summary for 2.6.1.1 extracted from

  • Yagi, T.; Toyosato, M.; Soda, K.
    Crystalline aspartate aminotransferase from Pseudomonas striata (1976), FEBS Lett., 61, 34-37.
    View publication on PubMed
Search for more literature for 2.6.1.1

Crystallization (Commentary)

Crystallization (Comment) Organism
concentrated protein solution, potassium phosphate 0,01 M, pH 7.2, 0.01 mM pyridoxal 5'-phosphate, ammonium sulfate Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 meniscus depletion sedimentation equilibrium method Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Pseudomonas putida enzyme plays the most important role in amino acid metabolism oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Pseudomonas putida IFO 12996 enzyme plays the most important role in amino acid metabolism oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida IFO 12996
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida
45.15
-
 purified enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate 34% of the activity with glutamate Pseudomonas putida oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate enzyme plays the most important role in amino acid metabolism Pseudomonas putida oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate 34% of the activity with glutamate Pseudomonas putida IFO 12996 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate enzyme plays the most important role in amino acid metabolism Pseudomonas putida IFO 12996 oxaloacetate + L-glutamate
-
 ?
L-cysteine sulfinic acid + 2-oxoglutarate 21% of the activity with L-glutamate Pseudomonas putida 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
L-cysteine sulfinic acid + 2-oxoglutarate 21% of the activity with L-glutamate Pseudomonas putida IFO 12996 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Pseudomonas putida 2-oxoglutarate + L-glutamate
-
 ?
L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Pseudomonas putida IFO 12996 2-oxoglutarate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate 6.9% of the activity with glutamate Pseudomonas putida 2-oxo-3-phenylpropionic acid + L-glutamate i.e. phenylpyruvate ?
L-tyrosine + 2-oxoglutarate 1.2% of the activity with glutamate Pseudomonas putida 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate i.e. 4-hydroxyphenylpyruvate ?
additional information substrate specificity Pseudomonas putida ?
-
 ?
additional information substrate specificity Pseudomonas putida IFO 12996 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.3 2 reaction methods Pseudomonas putida
8.3 8.6
-
 Pseudomonas putida