Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hadacidin | i.e. N-formylhydroxyaminoacetic acid | Atriplex spongiosa | |
malate | oxoglutarate production, oxaloacetate production | Atriplex spongiosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
oxaloacetate | isozyme Asp-DEAE-2, pH 8.0 | Atriplex spongiosa | |
0.08 | - |
2-oxoglutarate | isozyme Asp-DEAE-1, pH 8.0 | Atriplex spongiosa | |
0.1 | - |
oxaloacetate | isozyme Asp-DEAE-1, pH 8.0 | Atriplex spongiosa | |
0.12 | - |
2-oxoglutarate | isozyme Asp-DEAE-2, pH 8.0 | Atriplex spongiosa | |
0.5 | - |
L-aspartate | isozyme Asp-DEAE-2, pH 8.0 | Atriplex spongiosa | |
0.75 | - |
L-glutamate | isozyme Asp-DEAE-2, pH 8.0 | Atriplex spongiosa | |
0.85 | - |
L-aspartate | isozyme Asp-DEAE-1, pH 8.0 | Atriplex spongiosa | |
1.6 | - |
L-glutamate | isozyme Asp-DEAE-1, pH 8.0 | Atriplex spongiosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Atriplex spongiosa | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Atriplex spongiosa | - |
2 major isozymes: Asp-DEAE-1 and Asp-DEAE-2 | - |
Purification (Comment) | Organism |
---|---|
isoenzymes: Asp-DEAE-1 and Asp-DEAE-2 | Atriplex spongiosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bundle sheath | isozyme Asp-DEAE-2 | Atriplex spongiosa | - |
leaf | - |
Atriplex spongiosa | - |
mesophyll | isozyme Asp-DEAE-1 | Atriplex spongiosa | - |
Storage Stability | Organism |
---|---|
0°C, stable for at least several h in solution | Atriplex spongiosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Atriplex spongiosa | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | isozyme Asp-DEAE-1 performs preferably the reverse reaction, isozyme the forward reaction | Atriplex spongiosa | oxaloacetate + L-glutamate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.3 | formation of oxaloacetate, isoenzyme Asp-DEAE-1 | Atriplex spongiosa |
8 | - |
oxaloacetate formation, isoenzyme Asp-DEAE-2 | Atriplex spongiosa |