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Literature summary for 2.6.1.1 extracted from

  • Xing, R.; Whitman, W.B.
    Characterization of amino acid aminotransferases of Methanococcus aeolicus (1974), J. Bacteriol., 174, 541-548.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate in cell extracts, alanine activity is inhibited by 50% by about 2.5 mM 2-oxoglutarate, while the aspartate activity is unaffected at this concentration. The highly purified enzyme is also inhibited by 2-oxoglutarate, but the extent of inhibition is slightly more than that in extracts. Thus, alanine activity is completely inhibited by 10 mM 2-oxoglutarate, and 36% of the aspartate activity is inhibited Methanococcus aeolicus
L-aspartate inhibits tyrosine transamination Methanococcus aeolicus
L-cysteine 10 mM, 30% inactivation Methanococcus aeolicus
L-histidine inhibition is relieved by high concentrations of substrates Methanococcus aeolicus
additional information the activities with 2 mM aspartate or alanine are not inhibited by 10 mM isoleucine, phenylalanine, arginine, asparagine, glutamine, glycine, lysine, methionine, proline, serine, and threonine Methanococcus aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 2-oxoglutarate pH 7.5, 37°C Methanococcus aeolicus
0.5
-
 2-oxoglutarate pH 7.5, 37°C Methanococcus aeolicus
3.5
-
 L-alanine pH 7.5, 37°C Methanococcus aeolicus
3.7
-
 L-aspartate pH 7.5, 37°C Methanococcus aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
162000
-
 gel filtration Methanococcus aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Methanococcus aeolicus
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanococcus aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Methanococcus aeolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 pH 7.5, 37°C, enzyme in crude extract Methanococcus aeolicus
25.3
-
 pH 7.5, 37°C, partiall purified enzyme Methanococcus aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate 0.7% of the activity with L-aspartate Methanococcus aeolicus pyruvate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Methanococcus aeolicus oxaloacetate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate 0.07% of the activity with L-aspartate Methanococcus aeolicus 2-oxo-3-phenylpropionic acid + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate 0.08% of the activity with aspartate Methanococcus aeolicus 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate 0.08% of the activity with L-aspartate Methanococcus aeolicus 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate 0.04% of the activity with aspartate Methanococcus aeolicus 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate i.e. 4-hydroxyphenylpyruvate ?
L-tyrosine + 2-oxoglutarate 0.04% of the activity with L-aspartate Methanococcus aeolicus 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
aspartate aminotransferase
-
 Methanococcus aeolicus
AspAT
-
 Methanococcus aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanococcus aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Methanococcus aeolicus

pI Value

Organism Comment pI Value Maximum pI Value
Methanococcus aeolicus isoelectric focusing
-
 4.4