Cloned (Comment) | Organism |
---|---|
cloned into the expression vector pYE-express. The expression construct is transformed into Escherichia coli BL21 (DE3) | Fusarium fujikuroi |
Protein Variants | Comment | Organism |
---|---|---|
E109M | the variant shows less than 1% of the wild-type | Fusarium fujikuroi |
E90A | completely inactive enzyme | Fusarium fujikuroi |
F238R | the variant shows less than 2% residual catalytic efficiency of the wild-type | Fusarium fujikuroi |
I191Y | the variant shows less than 2% residual catalytic efficiency of the wild-type | Fusarium fujikuroi |
K187M | the variant shows diminished catalytic efficiency (kcat/Km) in comparison to the wild-type | Fusarium fujikuroi |
K253M | the variant shows diminished catalytic efficiency (kcat/Km) in comparison to the wild-type | Fusarium fujikuroi |
K332Q | the variant shows 50% catalytic efficiency of the wild-type enzyme | Fusarium fujikuroi |
R105K | the variant exhibits 9% of the catalytic efficiency of the wild-type | Fusarium fujikuroi |
R105M | the variant shows diminished catalytic efficiency (kcat/Km) in comparison to the wild-type | Fusarium fujikuroi |
R251K | the variant shows 58% catalytic efficiency of the wild-type enzyme | Fusarium fujikuroi |
R251M | the variant shows 21% catalytic efficiency of the wild-type enzyme | Fusarium fujikuroi |
R257L | the variant shows less than 2% residual catalytic efficiency of the wild-type | Fusarium fujikuroi |
S400Y | the variant shows 56% catalytic efficiency of the wild-type enzyme | Fusarium fujikuroi |
Y189F | the variant shows diminished catalytic efficiency (kcat/Km) in comparison to the wild-type | Fusarium fujikuroi |
Y255F | the variant shows diminished catalytic efficiency (kcat/Km) in comparison to the wild-type | Fusarium fujikuroi |
Y404F | the variant shows diminished catalytic efficiency (kcat/Km) in comparison to the wild-type | Fusarium fujikuroi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
Fusarium fujikuroi | |
Cu2+ | - |
Fusarium fujikuroi | |
geranyl diphosphate | strong substrate inhibition above concentrations of 0.005 mM | Fusarium fujikuroi | |
Mn2+ | - |
Fusarium fujikuroi | |
Ni2+ | - |
Fusarium fujikuroi | |
Zn2+ | - |
Fusarium fujikuroi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
geranyl diphosphate | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.31 | - |
L-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.34 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
3.4 | - |
tyrosine | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
5.61 | - |
5-hydroxy-L-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
7.94 | - |
D-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no dependency on divalent metal cations, but addition of Mg2+ and Ca2+ leads to slightly enhanced reaction rates | Fusarium fujikuroi | |
Mg2+ | no dependency on divalent metal cations, but addition of Mg2+ and Ca2+ leads to slightly enhanced reaction rates | Fusarium fujikuroi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | Fusarium fujikuroi | - |
diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | Fusarium fujikuroi IMI58289 | - |
diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium fujikuroi | - |
- |
- |
Fusarium fujikuroi IMI58289 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Fusarium fujikuroi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan | - |
Fusarium fujikuroi | diphosphate + 5-hydroxy-1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan | - |
Fusarium fujikuroi IMI58289 | diphosphate + 5-hydroxy-1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + D-tryptophan | - |
Fusarium fujikuroi | diphosphate + 1-(2-methylbut-3-en-2-yl)-D-tryptophan | - |
? | |
dimethylallyl diphosphate + D-tryptophan | - |
Fusarium fujikuroi IMI58289 | diphosphate + 1-(2-methylbut-3-en-2-yl)-D-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Fusarium fujikuroi | diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | the reaction catalyzed by the enzyme (DMATS1Ff) is nonstereospecific. No activity with isopentenyl diphosphate, farnesyl diphosphate and geranylgeranyl diphosphate | Fusarium fujikuroi | diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Fusarium fujikuroi IMI58289 | diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | the reaction catalyzed by the enzyme (DMATS1Ff) is nonstereospecific. No activity with isopentenyl diphosphate, farnesyl diphosphate and geranylgeranyl diphosphate | Fusarium fujikuroi IMI58289 | diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + tyrosine | - |
Fusarium fujikuroi | diphosphate + O-(3-methylbut-2-en-1-yl)-L-tyrosine | - |
? | |
dimethylallyl diphosphate + tyrosine | - |
Fusarium fujikuroi IMI58289 | diphosphate + O-(3-methylbut-2-en-1-yl)-L-tyrosine | - |
? | |
geranyl diphosphate + L-tryptophan | - |
Fusarium fujikuroi | diphosphate + 1-(3,7-dimethylocta-1,6-dien-3-yl)-L-tryptophan | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DMATS1Ff | - |
Fusarium fujikuroi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Fusarium fujikuroi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | 25°C: about 50% of maximal activity, 40°C: about 70% of maximal activity | Fusarium fujikuroi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00084 | - |
geranyl diphosphate | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.0038 | - |
D-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.0056 | - |
tyrosine | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.02 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme K253M | Fusarium fujikuroi | |
0.02 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme K253M | Fusarium fujikuroi | |
0.03 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme Y189F | Fusarium fujikuroi | |
0.03 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme Y189F | Fusarium fujikuroi | |
0.06 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme Y255F | Fusarium fujikuroi | |
0.06 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme Y255F | Fusarium fujikuroi | |
0.1 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme I191Y | Fusarium fujikuroi | |
0.1 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme I191Y | Fusarium fujikuroi | |
0.1 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme R105K | Fusarium fujikuroi | |
0.1 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme R105K | Fusarium fujikuroi | |
0.11 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme F238R | Fusarium fujikuroi | |
0.11 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme F238R | Fusarium fujikuroi | |
0.12 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme R251M | Fusarium fujikuroi | |
0.12 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme R251M | Fusarium fujikuroi | |
0.16 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme E109M | Fusarium fujikuroi | |
0.16 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme E109M | Fusarium fujikuroi | |
0.16 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme R105M | Fusarium fujikuroi | |
0.16 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme R105M | Fusarium fujikuroi | |
0.21 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme K187M | Fusarium fujikuroi | |
0.21 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme K187M | Fusarium fujikuroi | |
0.44 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme R257L | Fusarium fujikuroi | |
0.44 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme R257L | Fusarium fujikuroi | |
0.69 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.72 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme K332Q | Fusarium fujikuroi | |
0.72 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme K332Q | Fusarium fujikuroi | |
1.27 | - |
L-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
1.49 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme S400Y | Fusarium fujikuroi | |
1.49 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme S400Y | Fusarium fujikuroi | |
2.26 | - |
5-hydroxy-L-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
2.87 | - |
L-tryptophan | pH 7.5, 35°C, mutant enzyme R251K | Fusarium fujikuroi | |
2.87 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, mutant enzyme R251K | Fusarium fujikuroi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Fusarium fujikuroi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 55% of maximal activity, pH 9.0: about 90% of maximal activity | Fusarium fujikuroi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.278 | - |
geranyl diphosphate | pH 7.5, 35°C | Fusarium fujikuroi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00048 | - |
D-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.02 | - |
tyrosine | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.16 | - |
geranyl diphosphate | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
0.4 | - |
5-hydroxy-L-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
1.99 | - |
dimethylallyl diphosphate | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi | |
4.07 | - |
L-tryptophan | pH 7.5, 35°C, wild-type enzyme | Fusarium fujikuroi |