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Literature summary for 2.5.1.B43 extracted from

  • Hitchcock, A.; Jackson, P.J.; Chidgey, J.W.; Dickman, M.J.; Hunter, C.N.; Canniffe, D.P.
    Biosynthesis of chlorophyll a in a purple bacterial phototroph and assembly into a plant chlorophyll-protein complex (2016), ACS Synth. Biol., 5, 948-954.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information improvements to photosynthetic efficiency can be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption via development of organisms that can produce both bacteriochlorophylls and chlorophylls, engineering of the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Deletion of genes responsible for the bacteriochlorophyll-specific modifications of chlorophyllide and replacement of the native bacteriochlorophyll synthase with a cyanobacterial chlorophyll synthase results in the production of chlorophyll a. Chlorophyll a can be assembled in vivo into the plant water-soluble chlorophyll protein, heterologously produced in Rhodobacter sphaeroides, method optimization, overview. Mutant subcloning in Escherichia coli strains JM109 and S17-1 Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacteriochlorophyllide a + phytyl diphosphate Cereibacter sphaeroides
-
bacteriochlorophyll a + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides A4WRA3
-
-

Reaction

Reaction Comment Organism Reaction ID
phytyl diphosphate + bacteriochlorophyllide a = bacteriochlorophyll a + diphosphate the enzyme catalyses the direct addition of a phytyl chain to bacteriochlorophyllide a via an ester bond with the 17-propionate residue resulting in bacteriochlorophyll a Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriochlorophyllide a + phytyl diphosphate
-
Cereibacter sphaeroides bacteriochlorophyll a + diphosphate
-
?
additional information an engineered enzyme mutant is also active with chlorophyllide a and phytyl diphosphate producing chlorophyll a and diphosphate, cf. EC 2.5.1.62 Cereibacter sphaeroides ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Cereibacter sphaeroides

General Information

General Information Comment Organism
metabolism bacteriochlorophyll and chlorophyll share a common biosynthetic pathway up to the precursor chlorophyllide Cereibacter sphaeroides