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Literature summary for 2.5.1.B41 extracted from

  • Ueda, D.; Yamaga, H.; Murakami, M.; Totsuka, Y.; Shinada, T.; Sato, T.
    Biosynthesis of sesterterpenes, head-to-tail triterpenes, and sesquarterpenes in Bacillus clausii: identification of multifunctional enzymes and analysis of isoprenoid metabolites (2015), ChemBioChem, 16, 1371-1377.
    View publication on PubMed
Search for more literature for 2.5.1.B41

Cloned(Commentary)

Cloned (Comment) Organism
gene bcl-hept, DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged enzyme from pCold I vector as soluble protein in Escherichia coli strain BL21(DE3) Alkalihalobacillus clausii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Alkalihalobacillus clausii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate Alkalihalobacillus clausii heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate Alkalihalobacillus clausii JCM 9138 heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate Alkalihalobacillus clausii heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate Alkalihalobacillus clausii JCM 9138 heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + isopentenyl diphosphate Alkalihalobacillus clausii heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.81 (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?
additional information Alkalihalobacillus clausii formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate ?
-
 ?
additional information Alkalihalobacillus clausii JCM 9138 formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus clausii S6AGL5 gene bcl-hept
-
Alkalihalobacillus clausii S6B6P7 gene bcl-hept
-
Alkalihalobacillus clausii JCM 9138 S6AGL5 gene bcl-hept
-
Alkalihalobacillus clausii JCM 9138 S6B6P7 gene bcl-hept
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Alkalihalobacillus clausii

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate (3) Alkalihalobacillus clausii
a
geranylgeranyl diphosphate + 2 isopentenyl diphosphate = 2 diphosphate + all-trans-hexaprenyl diphosphate (2) Alkalihalobacillus clausii
a
geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate (1) Alkalihalobacillus clausii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity Alkalihalobacillus clausii 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30 Alkalihalobacillus clausii 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity Alkalihalobacillus clausii JCM 9138 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30 Alkalihalobacillus clausii JCM 9138 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 Alkalihalobacillus clausii 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 Alkalihalobacillus clausii JCM 9138 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + isopentenyl diphosphate heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.81 Alkalihalobacillus clausii (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?
additional information formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate Alkalihalobacillus clausii ?
-
 ?
additional information product identification and determination by mass spectrometry, NMR spectrometry, and TLC Alkalihalobacillus clausii ?
-
 ?
additional information formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate Alkalihalobacillus clausii JCM 9138 ?
-
 ?
additional information product identification and determination by mass spectrometry, NMR spectrometry, and TLC Alkalihalobacillus clausii JCM 9138 ?
-
 ?

Synonyms

Synonyms Comment Organism
(all-E)-isoprenyl diphosphate synthase
-
 Alkalihalobacillus clausii
E-IDS2-L
-
 Alkalihalobacillus clausii
E-IDS2-S
-
 Alkalihalobacillus clausii
geranylfarnesyl diphosphate/hexaprenyl diphosphate/heptaprenyl diphosphate synthase
-
 Alkalihalobacillus clausii
GFPP/HexPP/HepPP synthase
-
 Alkalihalobacillus clausii
HepS
-
 Alkalihalobacillus clausii
HepT
-
 Alkalihalobacillus clausii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Alkalihalobacillus clausii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Alkalihalobacillus clausii

General Information

General Information Comment Organism
metabolism hexaprenyl diphosphate and heptaprenyl diphosphate, intermediates of two isoprenoid pathways (acyclic terpenes and menaquinones), are biosynthesized by one trifunctional (all-E)-isoprenyl diphosphate synthase, E-IDS. Geranylfarnesyl diphosphate/hexaprenyl diphosphate and heptaprenyl diphosphate are the primary substrates for the biosynthesis of acyclic terpenes and menaquinone-7, respectively. (All-E)-and E,Z-mixed isoprenyl diphosphates are formed by E-IDSs and Z-IDSs, respectively, which have no sequence similarity with each other Alkalihalobacillus clausii
physiological function all-trans-heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), and is used to produce menaquinone-7 Alkalihalobacillus clausii