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Literature summary for 2.5.1.94 extracted from

  • Chankhamjon, P.; Boettger-Schmidt, D.; Scherlach, K.; Urbansky, B.; Lackner, G.; Kalb, D.; Dahse, H.M.; Hoffmeister, D.; Hertweck, C.
    Biosynthesis of the halogenated mycotoxin aspirochlorine in koji mold involves a cryptic amino acid conversion (2014), Angew. Chem. Int. Ed. Engl., 53, 13409-13413.
    View publication on PubMed
Search for more literature for 2.5.1.94

Cloned(Commentary)

Cloned (Comment) Organism
gene aclH, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
additional information construction of an aclH deletion mutant of Aspergillus oryzae RIB40 strain, that is inactive with 3-chloro-L-tyrosine and 3-chloro-DL-phenylalanine. The DaclH mutant culture accumulates dechloroaspirochlorine. Complementation by ectopic integration of aclH in the DaclH mutant restores aspirochlorine production Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
 gene aclH
-
Aspergillus oryzae RIB 40
-
 gene aclH
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + chloride
-
 Aspergillus oryzae 5'-deoxy-5'-chloroadenosine + L-methionine
-
 ?
S-adenosyl-L-methionine + chloride
-
 Aspergillus oryzae RIB 40 5'-deoxy-5'-chloroadenosine + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
AclH
-
 Aspergillus oryzae
chlorinase
-
 Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
flavin dependent on Aspergillus oryzae

General Information

General Information Comment Organism
metabolism the enzyme catalyzes chlorination, the last step in the aspirochlorine pathway, modeling of the aspirochlorine biosynthesis involving an unusual Phe to Gly conversion, overview Aspergillus oryzae
physiological function key role of AclH as a chlorinase in the aspirochlorine biosynthesis. Aspirochlorine, or antibiotic A30641, is an epidithiodiketopiperazine toxin distinctive antifungal properties due to selective inhibition of protein biosynthesis produced from Aspergillus oryzae Aspergillus oryzae