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Literature summary for 2.5.1.93 extracted from

  • Mühlenweg, A.; Melzer, M.; Li, S.M.; Heide, L.
    4-Hydroxybenzoate 3-geranyltransferase from Lithospermum erythrorhizon: purification of a plant membrane-bound prenyltransferase (1998), Planta, 205, 407-413.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-chloro-mercuriphenylsulfonic acid 0.1 mM, completely inhibits 4HB geranyltransferase activity Lithospermum erythrorhizon
Digitonin
-
Lithospermum erythrorhizon
iodoacetamide 10 mM, completely inhibits 4HB geranyltransferase activity Lithospermum erythrorhizon
N-Methylmaleimide 10 mM, completely inhibits 4HB geranyltransferase activity Lithospermum erythrorhizon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0138
-
geranyl diphosphate pH 7.8, 37°C Lithospermum erythrorhizon
0.0184
-
4-hydroxybenzoate pH 7.8, 37°C Lithospermum erythrorhizon

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal concentration: 20-50 mM Lithospermum erythrorhizon

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
341000
-
gel filtration Lithospermum erythrorhizon

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + 4-hydroxybenzoate Lithospermum erythrorhizon regulatory enzyme in the biosynthesis of shikonin 3-geranyl-4-hydroxybenzoate + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Lithospermum erythrorhizon
-
Sieb. et Zucc.
-

Purification (Commentary)

Purification (Comment) Organism
Of various detergents examined, digitonin is the most suitable for the solubilization of the enzyme Lithospermum erythrorhizon

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Lithospermum erythrorhizon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.32
-
-
Lithospermum erythrorhizon

Storage Stability

Storage Stability Organism
-20°C, in the presence of 10% glycerol, frozen enzyme preparations are stable upon storage for at least two months. Protease inhibitors directed against serin-, carboxyl-, and metalloproteases such as PMSF, 1,2-epoxy-3-(4-nitrophenoxy)-propane or EGTA do not increase the stability of the enzyme solution. Lithospermum erythrorhizon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + 4-hydroxybenzoate regulatory enzyme in the biosynthesis of shikonin Lithospermum erythrorhizon 3-geranyl-4-hydroxybenzoate + diphosphate
-
?
geranyl diphosphate + 4-hydroxybenzoate the enzyme is highly specific for geranyl diphosphate and 4-hydroxybenzoate Lithospermum erythrorhizon 3-geranyl-4-hydroxybenzoate + diphosphate
-
?

Synonyms

Synonyms Comment Organism
4HB geranyltransferase
-
Lithospermum erythrorhizon

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
46
-
-
Lithospermum erythrorhizon

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 63 half-maximal activity at 35°C and at 63°C Lithospermum erythrorhizon

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
in Bis-Tris-HCl buffer Lithospermum erythrorhizon
8.5
-
in Tris-HCl buffer Lithospermum erythrorhizon

pH Range

pH Minimum pH Maximum Comment Organism
6.2 9.3 half-maximal activity at pH 6.2 and at pH 9.3, Tris-HCl buffer Lithospermum erythrorhizon

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
at pH 4 or 8, all activtiy is lost quickly Lithospermum erythrorhizon
5
-
storage at pH 5 or pH 7 results in a loss of activity between 20% and 30% in 8 days Lithospermum erythrorhizon
6
-
at pH 6, the enzyme can be stored in the presence of 1.5 mM digitonin, 10% glycerol and 2 mM DTT at 4°C for two weeks with minimal loss of activity Lithospermum erythrorhizon
7
-
storage at pH 5 or pH 7 results in a loss of activity between 20% and 30% in 8 days Lithospermum erythrorhizon
8
-
at pH 4 or 8, all activtiy is lost quickly Lithospermum erythrorhizon

General Information

General Information Comment Organism
physiological function regulatory enzyme in the biosynthesis of shikonin Lithospermum erythrorhizon