Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.91 extracted from

  • Ishii, K.; Sagami, H.; Ogura, K.
    Decaprenyl pyrophosphate synthetase from Paracoccus denitrificans (1985), Biochim. Biophys. Acta, 835, 291-297.
No PubMed abstract available
Search for more literature for 2.5.1.91

Activating Compound

Activating Compound Comment Organism Structure
NH4+ activation Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00006
-
 (2E,6E)-farnesyl diphosphate pH 7.3, 37°C Paracoccus denitrificans
0.0029
-
 all-E-geranylgeranyl diphosphate pH 7.3, 37°C Paracoccus denitrificans
0.005
-
 geranyl diphosphate pH 7.3, 37°C Paracoccus denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation Paracoccus denitrificans
Mg2+ required. The relative amount of C50 and C55 products, i.e. decaprenyl diphosphate and undecaprenyl diphosphate, changes depending on the concentration of Mg2+. The ratio of the C50 and C55 product formed at 1 mM Mg2+ is 23 : 1 Paracoccus denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Paracoccus denitrificans 7 diphosphate + all-trans-decaprenyl diphosphate plus minor amounts of undecaprenyl diphosphate ?
all-E-geranylgeranyl diphosphate + isopentenyl diphosphate 74% of the activity with (2E,6E)-farnesyl diphosphate Paracoccus denitrificans diphosphate + all-trans-decaprenyl diphosphate plus minor amounts of undecaprenyl diphosphate ?
geranyl diphosphate + 6 isopentenyl diphosphate 95% of the activity with E,E-farnesyl diphosphate Paracoccus denitrificans diphosphate + all-trans-decaprenyl diphosphate the relative amount of C50 and C55 products, i.e. decaprenyl diphosphate and undecaprenyl diphosphate, changes depending on the concentration of Mg2+. The ratio of the C50 and C55 product formed at 1 mM Mg2+ is 23:1 ?
additional information no substrate: dimethylallyl diphosphate Paracoccus denitrificans ?
-
 ?