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Literature summary for 2.5.1.91 extracted from

  • Saiki, R.; Nagata, A.; Uchida, N.; Kainou, T.; Matsuda, H.; Kawamukai, M.
    Fission yeast decaprenyl diphosphate synthase consists of Dps1 and the newly characterized Dlp1 protein in a novel heterotetrameric structure (2003), Eur. J. Biochem., 270, 4113-4121.
    View publication on PubMed
Search for more literature for 2.5.1.91

Cloned(Commentary)

Cloned (Comment) Organism
the proteins Dps1 abd Dlp1 must be present simultaneously in Escherichia coli transformants before ubiquinone-10, which is produced by Schizosaccharomyces pombe but not by Escherichia coli, is generated. DLP1 and DPS1 form a heterotetramer in Escherichia coli Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 gel filtration Schizosaccharomyces pombe
32411
-
 2 * 42047 (DSP1) + 2 * 32411 (DLP1), both DPS1 and DLPd1 are required for the enzymatic activity, calculated from sequence Schizosaccharomyces pombe
42047
-
 2 * 42047 (DSP1) + 2 * 32411 (DLP1), both DPS1 and DLPd1 are required for the enzymatic activity, calculated from sequence Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O13851 gene DLP1
-
Schizosaccharomyces pombe O43091 gene DPS1
-
Schizosaccharomyces pombe O43091 and O13851 O43091: subunit 1 (DSP1), O13851: subunit 2 (DLP1)
-

Subunits

Subunits Comment Organism
heterotetramer 2 * 42047 (DSP1) + 2 * 32411 (DLP1), both DPS1 and DLPd1 are required for the enzymatic activity, calculated from sequence Schizosaccharomyces pombe
More both Dps1 and Dlp1 are required for enzymatic activity Schizosaccharomyces pombe
tetramer 2 * Dps1 + 2 * Dlp1, heterotetramer Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
D-less polyprenyl diphosphate synthase
-
 Schizosaccharomyces pombe
decaprenyl diphosphate synthase
-
 Schizosaccharomyces pombe
DLP1
-
 Schizosaccharomyces pombe
DPS1
-
 Schizosaccharomyces pombe

General Information

General Information Comment Organism
malfunction the deletion mutant of dlp1 lacks the enzymatic activity of decaprenyl diphosphate synthase, does not produce ubiquinone-10 and has the typical ubiquinone-deficient Schizosaccharomyces pombe phenotypes, namely hypersensitivity to hydrogen peroxide, the need for antioxidants for growth on minimal medium and an elevated production of H2S. Both the dps1 and dlp1 mutants can generate ubiquinone when they are transformed with a bacterial decaprenyl diphosphate synthase, which functions in its host as a homodimer Schizosaccharomyces pombe
physiological function both genes DPS1 and DLP1 are required for decaprenyl diphosphate synthase activity. The deletion mutant of dlp1 lacks the enzymatic activity of decaprenyl diphosphate synthase, does not produce ubiquinone-10 and has the typical ubiquinone-deficient Schizosaccharomyces pombe phenotypes, namely hypersensitivity to hydrogen peroxide, the need for antioxidants for growth on minimal medium and an elevated production of H2S. Both the dps1 and dlp1 mutants can generate ubiquinone when they are transformed with a bacterial decaprenyl diphosphate synthase, which functions in its host as a homodimer Schizosaccharomyces pombe