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Literature summary for 2.5.1.91 extracted from

  • Lee, J.K.; Her, G.; Kim, S.Y.; Seo, J.H.
    Cloning and functional expression of the dps gene encoding decaprenyl diphosphate synthase from Agrobacterium tumefaciens (2004), Biotechnol. Prog., 20, 51-56.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Escherichia coli JM109 harboring the dps gene produces ubiquinone-10 in addition to endogenous ubiquinone-8 Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
D245E significant decrease of activity Agrobacterium tumefaciens
D245G complete loss of activity Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36400
-
 2 * 36400, calculated from sequence Agrobacterium tumefaciens
38960
-
 2 * 38960, calculated from sequence Agrobacterium tumefaciens
71000
-
 gel filtration Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens Q5QKG1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Agrobacterium tumefaciens

Subunits

Subunits Comment Organism
homodimer 2 * 36400, calculated from sequence Agrobacterium tumefaciens
homodimer 2 * 38960, calculated from sequence Agrobacterium tumefaciens

pI Value

Organism Comment pI Value Maximum pI Value
Agrobacterium tumefaciens calculated from sequence
-
 5.16

Expression

Organism Comment Expression
Agrobacterium tumefaciens the recombinant enzyme is expressed with various concentrations of IPTG at various temperatures. A significant level of expression is observed when the enzyme is induced at 20°C with 0.25 mM isopropyl-beta-D-thiogalactopyranoside up