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Literature summary for 2.5.1.90 extracted from

  • Takahashi, H.; Aihara, Y.; Ogawa, Y.; Murata, Y.; Nakajima, K.I.; Iida, M.; Shirai, M.; Fujisaki, S.
    Suppression of phenotype of Escherichia coli mutant defective in farnesyl diphosphate synthase by overexpression of gene for octaprenyl diphosphate synthase (2018), Biosci. Biotechnol. Biochem., 82, 1003-1010 .
    View publication on PubMed
Search for more literature for 2.5.1.90

Cloned(Commentary)

Cloned (Comment) Organism
hexahistidine-tagged octaprenyl diphosphate synthase Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 dimethylallyl diphosphate at pH 7.5 and 30°C Escherichia coli
0.34
-
 isopentenyl diphosphate with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM used in assay conditions Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate Escherichia coli
-
 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate Escherichia coli K12
-
 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AD57
-
-
Escherichia coli P0AD57 K12 strain SF5
-
Escherichia coli K12 P0AD57 K12 strain SF5
-

Purification (Commentary)

Purification (Comment) Organism
hexahistidine-tagged octaprenyl diphosphate synthase Escherichia coli
Ni-NTA agarose column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
430
-
 with dimethylallyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli
3100
-
 with geranyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli
31000
-
 with geranylgeranyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli
58000
-
 with (2E,6E)-farnesyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate (2E,6E)-farnesyl diphosphate is the most effective substrate Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli K12 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate (2E,6E)-farnesyl diphosphate is the most effective substrate Escherichia coli K12 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
dimethylallyl diphosphate + isopentenyl diphosphate 0.7% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate
-
 ?
dimethylallyl diphosphate + isopentenyl diphosphate 0.7% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli K12 diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate 5.3% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli ?
-
 ?
geranyl diphosphate + isopentenyl diphosphate 5.3% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli K12 ?
-
 ?
geranylgeranyl diphosphate + isopentenyl diphosphate 53.4% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli ?
-
 ?
geranylgeranyl diphosphate + isopentenyl diphosphate 53.4% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli K12 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
IspB
-
 Escherichia coli
octaprenyl diphosphate synthase
-
 Escherichia coli