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Literature summary for 2.5.1.90 extracted from

  • Teng, K.H.; Hsu, E.T.; Chang, Y.H.; Lin, S.W.; Liang, P.H.
    Fluorescent farnesyl diphosphate analogue A probe to validate trans-prenyltransferase inhibitors (2016), Biochemistry, 55, 4366-4374 .
    View publication on PubMed
Search for more literature for 2.5.1.90

Inhibitors

Inhibitors Comment Organism Structure
zoledronate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate at pH 7.5, temperature not specified in the publication Escherichia coli
0.0015
-
 (2E,6E)-farnesyl diphosphate at pH 7.5, temperature not specified in the publication Escherichia coli
0.004
-
 isopentenyl diphosphate with (2R,6E)-farnesyl diphosphate as cosubstrate, at pH 7.5, temperature not specified in the publication Escherichia coli
0.0069
-
 isopentenyl diphosphate with (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate as cosubstrate, at pH 7.5, temperature not specified in the publication Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM used in assay conditions Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate Escherichia coli
-
 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AD57
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli ?
-
 ?
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
long-chain C40 octaprenyl diphosphate synthase
-
 Escherichia coli
OPPs
-
 Escherichia coli
trans-prenyltransferase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.055
-
 (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate at pH 7.5, temperature not specified in the publication Escherichia coli
0.06
-
 (2E,6E)-farnesyl diphosphate at pH 7.5, temperature not specified in the publication Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00022
-
 at pH 7.5, temperature not specified in the publication Escherichia coli zoledronate