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Literature summary for 2.5.1.90 extracted from
- Reaction kinetic pathway of the recombinant octaprenyl pyrophosphate synthase from Thermotoga maritima How is it different from that of the mesophilic enzyme (2002), Biochim. Biophys. Acta, 1599, 125-133 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Thermotoga maritima |  |
| 0.002 | - |
isopentenyl diphosphate | pH 7.5, 25°C | Thermotoga maritima | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Thermotoga maritima | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Thermotoga maritima DSM 3109 | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9X1M1 | - |
- |
| Thermotoga maritima DSM 3109 | Q9X1M1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | Thermotoga maritima | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the product of chain elongation catalyzed by the Thermotoga maritima enzyme is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM octaprenyl pyrophosphate synthase/farnesyl pyrophosphate complex and 0.001 mM isopentenyl pyrophosphate, only the C20 is formed | Thermotoga maritima | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | Thermotoga maritima DSM 3109 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the product of chain elongation catalyzed by the Thermotoga maritima enzyme is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM octaprenyl pyrophosphate synthase/farnesyl pyrophosphate complex and 0.001 mM isopentenyl pyrophosphate, only the C20 is formed | Thermotoga maritima DSM 3109 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| octaprenyl pyrophosphate synthase | - |
Thermotoga maritima |
| OPPs | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 85 | the enzyme activity at temperatures ranging from 25°C to 85°C exponentially increases with elevated temperature | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
Tm-value is above 80°C | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Thermotoga maritima | |
| 0.005 | - |
isopentenyl diphosphate | pH 7.5, 25°C | Thermotoga maritima | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | octaprenyl pyrophosphate synthase catalyzes the chain elongation of farnesyl pyrophosphate via consecutive condensation reactions with five molecules of isopentenyl pyrophosphate to generate all-trans C40-octaprenyl pyrophosphate. The polymer forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | Thermotoga maritima |
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