Literature summary for 2.5.1.9 extracted from

Serer, M.I.; Bonomi, H.R.; Guimaraes, B.G.; Rossi, R.C.; Goldbaum, F.A.; Klinke, S.
Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility (2014), Acta Crystallogr. Sect. D, 70, 1419-1434.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Brucella abortus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 12% (w/v) PEG 8000, 10% (w/v) glycerol, 0.5 M potassium chloride	Brucella abortus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
66000	-	-	Brucella abortus
69800	-	-	Brucella abortus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine	Brucella abortus	-	riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?
6,7-dimethyl-8-(1-D-ribityl)lumazine	Brucella abortus 2308	-	riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?

Organism

Organism	UniProt	Comment	Textmining
Brucella abortus	Q2YN92	-	-
Brucella abortus 2308	Q2YN92	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex 200 gel filtration	Brucella abortus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine	-	Brucella abortus	riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?
6,7-dimethyl-8-(1-D-ribityl)lumazine	-	Brucella abortus 2308	riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 66000, static light scattering	Brucella abortus
homotrimer	3 * 69800, calculated from amino acid sequence	Brucella abortus

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Release 2023.1 (January 2023)