Literature summary for 2.5.1.9 extracted from

Illarionov, B.; Kemter, K.; Eberhardt, S.; Richter, G.; Cushman, M.; Bacher, A.
Riboflavin synthase of Escherichia coli. Effect of single amino acid substitutions on reaction rate and ligand binding properties (2001), J. Biol. Chem., 276, 11524-11530.

Cloned(Commentary)

Cloned (Comment)	Organism
ribC hyperexpression strain, DNA sequence determination of mutants	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D143G	site-directed mutagenesis, soluble protein, too unstable to be purified	Escherichia coli
D143N	site-directed mutagenesis, soluble protein, too unstable to be purified	Escherichia coli
D185L	site-directed mutagenesis, low remaining activity	Escherichia coli
E183G	site-directed mutagenesis, reduced activity	Escherichia coli
E66G	site-directed mutagenesis, low remaining activity	Escherichia coli
E85G	site-directed mutagenesis, reduced activity	Escherichia coli
F2A	site-directed mutagenesis, no remaining activity	Escherichia coli
F2Y	site-directed mutagenesis, very low remaining activity	Escherichia coli
H102Q	site-directed mutagenesis, very low remaining activity	Escherichia coli
H97Q	site-directed mutagenesis, low remaining activity	Escherichia coli
K137A	site-directed mutagenesis, low remaining activity	Escherichia coli
additional information	5 mutants genes cannot be expressed recombinantly in Escherichia coli: C48S, T50R, T67R, T148R, T165R	Escherichia coli
additional information	a F2DELTA deletion mutant construct has no remaining activity	Escherichia coli
N181G	site-directed mutagenesis, soluble protein, too unstable to be purified	Escherichia coli
N45G	site-directed mutagenesis, slightly reduced activity	Escherichia coli
N83G	site-directed mutagenesis, reduced activity	Escherichia coli
S146G	site-directed mutagenesis, low remaining activity	Escherichia coli
S41A	site-directed mutagenesis, very low remaining activity	Escherichia coli
T3R	site-directed mutagenesis, slightly reduced activity, low expression rate	Escherichia coli
T71A	site-directed mutagenesis, slightly reduced activity	Escherichia coli
Y133A	site-directed mutagenesis, soluble protein, too unstable to be purified	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine	Escherichia coli	-	riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	gene ribC	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type enzyme and mutants	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	reaction mechanism	Escherichia coli	a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	substrate binding site	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine	-	Escherichia coli	riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?

Subunits

Subunits	Comment	Organism
More	N-terminal structure, ligand binding site	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00833	-	6,7-dimethyl-8-ribityllumazine	per subunit	Escherichia coli