Cloned (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
E68A | product distributions is shifted to longer region to give the C65C70 as the main products | Thermococcus kodakarensis |
K109A | product distributions is shifted to longer region to give the C65C70 as the main products | Thermococcus kodakarensis |
L113A | product distributions is shifted to longer region to give the C65C70 as the main products | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00096 | - |
farnesyl diphosphate | 65°C | Thermococcus kodakarensis | |
0.00175 | - |
geranyl diphosphate | 65°C | Thermococcus kodakarensis | |
0.00588 | - |
isopentenyl diphosphate | 65°C, cosubstrate: geranyl diphosphate | Thermococcus kodakarensis | |
0.0094 | - |
dimethylallyl diphosphate | 65°C | Thermococcus kodakarensis | |
0.0132 | - |
isopentenyl diphosphate | 65°C, cosubstrate: farnesyl diphosphate | Thermococcus kodakarensis | |
0.111 | - |
isopentenyl diphosphate | 65°C, cosubstrate: dimethylallyl diphosphate | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | Thermococcus kodakarensis | the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate | n diphosphate + di-trans,poly-cis-polyprenyl diphosphate | - |
? | |
geranyl diphosphate + n isopentenyl diphosphate | Thermococcus kodakarensis | the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate | n diphosphate + di-trans,poly-cis-polyprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate | Thermococcus kodakarensis | n diphosphate + di-trans,poly-cis-polyprenyl diphosphate | - |
? | |
dimethylallyl diphosphate + n isopentenyl diphosphate | weak activity | Thermococcus kodakarensis | n diphosphate + di-trans,poly-cis-polyprenyl diphosphate | - |
? | |
geranyl diphosphate + n isopentenyl diphosphate | the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate | Thermococcus kodakarensis | n diphosphate + di-trans,poly-cis-polyprenyl diphosphate | - |
? | |
geranyl diphosphate + n isopentenyl diphosphate | the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate | Thermococcus kodakarensis | n diphosphate + di-trans,poly-cis-polyprenyl diphosphate | Tk-IdsB produces cis-polyprenyl diphosphates ranging from C15 to C90. Above C75: 11.5%. C65C70: 60.7%. C55C60: 17.4%. C45C50: 3.3%. C30C40: 3.9%. Below C25: 3.2%, mainly yields the C60C65 products | ? |
Synonyms | Comment | Organism |
---|---|---|
Tk-IdsB | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | pH 8.5 | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 46 min, half-life is prolonged by immobilization on silica beads to 156 min | Thermococcus kodakarensis |
96 | - |
not completely denatured | Thermococcus kodakarensis |