Literature summary for 2.5.1.86 extracted from

Ko, T.P.; Xiao, X.; Guo, R.T.; Huang, J.W.; Liu, W.; Chen, C.C.
Substrate-analogue complex structure of Mycobacterium tuberculosis decaprenyl diphosphate synthase (2019), Acta Crystallogr. Sect. F, 75, 212-216 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Mycobacterium tuberculosis
expression in Escherichia coli	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with the substrate analogues geranyl S-thiodiphosphate and isopentenyl S-thiodiphosphate, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 10% (v/v) glycerol, 25% (w/v) PEG 400, 7% (w/v) PEG 3000	Mycobacterium tuberculosis
sitting-drop vapour-diffusion method at 25°C. A 1.55 A resolution structure of the enzyme in complex with the substrate analogues geranyl S-thiodiphosphate and isopentenyl S-thiodiphosphate bound to their respective sites in one subunit shows the active-site configuration and the magnesium-coordinated geometry for catalysis	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	contains Mg2+	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	Mycobacterium tuberculosis	-	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	Mycobacterium tuberculosis H37Rv	-	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	Mycobacterium tuberculosis ATCC 25618	-	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WFF7	-	-
Mycobacterium tuberculosis ATCC 25618	P9WFF7	-	-
Mycobacterium tuberculosis H37Rv	P9WFF7	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and HiPrep 16/60 S-100 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	-	Mycobacterium tuberculosis	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	-	Mycobacterium tuberculosis H37Rv	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	-	Mycobacterium tuberculosis ATCC 25618	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
decaprenyl diphosphate synthase	-	Mycobacterium tuberculosis
DPPS	-	Mycobacterium tuberculosis
MtDPPS	-	Mycobacterium tuberculosis
Rv2361c	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)