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Literature summary for 2.5.1.86 extracted from

  • Zhang, H.; Li, Z.X.
    In vitro and in vivo characterization of a novel insect decaprenyl diphosphate synthase: a two-major step catalytic mechanism is proposed (2013), Biochem. Biophys. Res. Commun., 442, 105-111.
    View publication on PubMed
Search for more literature for 2.5.1.86

Cloned(Commentary)

Cloned (Comment) Organism
gene AgDPPS1, two cDNAs encoding the two subunits of an aphid long-chain PDDS, designated as AgDPPS1 and AgDPPS2, AgDPPS1 encodes subunit 1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of GST-tagged subunit in Escherichia coli strain BL21(DE3) Aphis gossypii
gene AgDPPS2, two cDNAs encoding the two subunits of an aphid long-chain PDDS, designated as AgDPPS1 and AgDPPS2, AgDPPS2 encodes subunit 2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of GST-tagged subunit in Escherichia coli strain BL21(DE3) Aphis gossypii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aphis gossypii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Aphis gossypii
-
 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aphis gossypii M9VRD0 gene AgDPPS2 encoding subunit 2
-
Aphis gossypii M9VU49 gene AgDPPS1 encoding subunit 1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged subunit from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, tag cleavage with factor Xa, and ultrafiltration Aphis gossypii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Aphis gossypii 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate enzyme DPPS accepts farnesyl diphosphate but not dimethylallyl diphosphate as the allylic co-substrate Aphis gossypii 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
additional information product identification by GC–MS analysis Aphis gossypii ?
-
 ?

Subunits

Subunits Comment Organism
dimer two cDNAs encoding the two subunits of an aphid long-chain PDDS, designated as AgDPPS1 and AgDPPS2 Aphis gossypii

Synonyms

Synonyms Comment Organism
decaprenyl diphosphate synthase 1
-
 Aphis gossypii
decaprenyl diphosphate synthase 2
-
 Aphis gossypii
DPP synthase
-
 Aphis gossypii
DPPS
-
 Aphis gossypii
polyprenyl diphosphate synthase
-
 Aphis gossypii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Aphis gossypii

pI Value

Organism Comment pI Value Maximum pI Value
Aphis gossypii sequence calculation
-
 6.28
Aphis gossypii sequence calculation
-
 8.13

General Information

General Information Comment Organism
physiological function the two subunits are essential for the activity of the enzyme. The insect enzyme is a DPP synthase with a two-major step catalytic mechanism, which catalyzes the formation of decaprenyl diphosphate as the final product, with geranylgeranyl diphosphate as the major intermediate product. The insect long-chain DPPS synthesizes the side-chain of coenzyme ubiquinone 10 Aphis gossypii