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Literature summary for 2.5.1.85 extracted from

  • Hirooka, K.; Izumi, Y.; An, C.I.; Nakazawa, Y.; Fukusaki, E.; Kobayashi, A.
    Functional analysis of two solanesyl diphosphate synthases from Arabidopsis thaliana (2005), Biosci. Biotechnol. Biochem., 69, 592-601.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, His6-tagged At-SPS2 fusion protein and the truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000565
-
geranylgeranyl diphosphate pH 8.0, 30°C, His6-tagged At-SPS2 fusion protein Arabidopsis thaliana
0.000843
-
geranylgeranyl diphosphate pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
0.00512
-
farnesyl diphosphate pH 8.0, 30°C, His6-tagged At-SPS2 fusion protein Arabidopsis thaliana
0.00689
-
(2E,6E)-farnesyl diphosphate pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
0.0289
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
0.0377
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, His6-tagged At-SPS2 fusion protein Arabidopsis thaliana
0.182
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
0.248
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, His6-tagged At-SPS2 fusion protein Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast At-SPS2 is transported into chloroplasts Arabidopsis thaliana 9507
-
endoplasmic reticulum At-SPS1 Arabidopsis thaliana 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46044
-
x * 46044, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + 5 isopentenyl diphosphate Arabidopsis thaliana At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana 5 diphosphate + all-trans-nonaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate Arabidopsis thaliana At-SPS2 supplies the precursor of the plastoquinone side-chain in Arabidopsis thaliana 5 diphosphate + all-trans-nonaprenyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q76FS5
-
-
Arabidopsis thaliana Q8S948
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged At-SPS2 fusion protein partially purified to about 10% purity, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) is purified to about 90% purity Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf mRNA level is 11fold higher in leaf than in root Arabidopsis thaliana
-
leaf mRNA level is 5fold higher in leaf than in root Arabidopsis thaliana
-
root mRNA level is 11fold higher in leaf than in root Arabidopsis thaliana
-
root mRNA level is 5fold higher in leaf than in root Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate At-SPS2 prefers geranylgeranyl diphosphate to farnesyl diphosphate as the allylic substrate Arabidopsis thaliana 6 diphosphate + all-trans-nonaprenyl diphosphate the main product is nonaprenyl diphosphate, considerable amounts of intermediates, such as geranylgeranyl diphosphate are formed ?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana Arabidopsis thaliana 5 diphosphate + all-trans-nonaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate At-SPS2 supplies the precursor of the plastoquinone side-chain in Arabidopsis thaliana Arabidopsis thaliana 5 diphosphate + all-trans-nonaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate At-SPS2 prefers geranylgeranyl diphosphate to farnesyl diphosphate as the allylic substrate Arabidopsis thaliana 5 diphosphate + all-trans-nonaprenyl diphosphate a large amount of nonaprenyl diphosphate is formed ?
additional information no activity with dimethylallyl diphosphate and geranyl diphosphate, recombinant His6-tagged At-SPS2 fusion protein and truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 46044, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At-SPS1
-
Arabidopsis thaliana
At-SPS2
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.72
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
2.33
-
geranylgeranyl diphosphate pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
2.83
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
3.77
-
(2E,6E)-farnesyl diphosphate pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.5
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
547
-
(2E,6E)-farnesyl diphosphate pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
595
-
isopentenyl diphosphate pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana
2770
-
geranylgeranyl diphosphate pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) Arabidopsis thaliana