Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli as as His6-tagged fusion protein | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.61 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
5.73 | - |
(2E,6E)-farnesyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
20 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate | Arabidopsis thaliana | |
151 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46600 | - |
2 * 46600, His6-tagged protein, calculated from sequence | Arabidopsis thaliana |
108000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8S948 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | geranylgeranyl diphosphate is preferred over farnesyl diphosphate as allylic substrate | Arabidopsis thaliana | 6 diphosphate + all-trans-nonaprenyl diphosphate | the main product is nonaprenyl diphosphate, considerable amounts of geranylgeranyl diphosphate are formed | ? | |
geranylgeranyl diphosphate + 5 isopentenyl diphosphate | geranylgeranyl diphosphate is preferred over farnesyl diphosphate as allylic substrate | Arabidopsis thaliana | 5 diphosphate + all-trans-nonaprenyl diphosphate | nonaprenyl diphosphate is almost exclusively formed | ? | |
additional information | no activity with farnesyl diphosphate and dimethylallyl diphosphate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46600, His6-tagged protein, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
At-SPS1 | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 8.0, 10 minm, stable below | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.7 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate | Arabidopsis thaliana | |
15 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate | Arabidopsis thaliana | |
21 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
29.7 | - |
farnesyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0968 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate | Arabidopsis thaliana | |
0.752 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate | Arabidopsis thaliana | |
5.19 | - |
(2E,6E)-farnesyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
13.1 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana |