Protein Variants | Comment | Organism |
---|---|---|
A79F | mutant enzyme subunit-A(wild-type)/subunit-B(A79L) shows 10fold increased Vmax-values and 11fold decreased Km-values for geranyl diphosphate, which becomes the most preferred substrate of the allylic primers. 5fold increase in KM-value for geranylgeranyl diphosphate. Mutation results in shortening the chain length of the major product. The major products are farnesylgeranyl diphosphate and geranylgeranyl diphosphate | Micrococcus luteus |
A79L | mutant enzyme subunit-A(wild-type)/subunit-B(A79L) shows 7fold increased Vmax-values and 6fold decreased Km-values for geranyl diphosphate, which becomes the most preferred substrate of the allylic primers. 3.9fold increase in KM-value for geranylgeranyl diphosphate. Mutation results in shortening the chain length of the major product. The major product is farnesylgeranyl diphosphate | Micrococcus luteus |
additional information | several amino acid residues in the larger subunits Bacillus subtilis heptaprenyl diphosphate synthase are selected for substitutions by site-directed mutagenesis and examined by combination with the corresponding wild type or mutated smaller subunits | Micrococcus luteus |
V76G | mutant enzyme subunit-A(wild-type)/subunit-B(V76G) gives octaprenyl diphosphate as the final products with farnesyl diphosphate as an allylic primer | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) | Micrococcus luteus | |
0.0075 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) | Micrococcus luteus | |
0.0089 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Micrococcus luteus | |
0.0105 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) | Micrococcus luteus | |
0.0124 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Micrococcus luteus | |
0.0137 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) | Micrococcus luteus | |
0.0184 | - |
isopentenyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Micrococcus luteus | |
0.0186 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) | Micrococcus luteus | |
0.0216 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) | Micrococcus luteus | |
0.0216 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) | Micrococcus luteus | |
0.0478 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) | Micrococcus luteus | |
0.385 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) | Micrococcus luteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | O66127 and O66129 | O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase | - |
Micrococcus luteus B-P 26 | O66127 and O66129 | O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate | - |
Micrococcus luteus | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranyl diphosphate + 4 isopentenyl diphosphate | geranyl diphosphate is a poor substrate | Micrococcus luteus | 4 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranyl diphosphate + 4 isopentenyl diphosphate | geranyl diphosphate is a poor substrate | Micrococcus luteus B-P 26 | 4 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | - |
Micrococcus luteus | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HexPP synthase | - |
Micrococcus luteus |