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Literature summary for 2.5.1.83 extracted from

  • Zhang, Y.W.; Li, X.Y.; Koyama, T.
    Chain length determination of prenyltransferases: both heteromeric subunits of medium-chain (E)-prenyl diphosphate synthase are involved in the product chain length determination (2000), Biochemistry, 39, 12717-12722.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A79F mutant enzyme subunit-A(wild-type)/subunit-B(A79L) shows 10fold increased Vmax-values and 11fold decreased Km-values for geranyl diphosphate, which becomes the most preferred substrate of the allylic primers. 5fold increase in KM-value for geranylgeranyl diphosphate. Mutation results in shortening the chain length of the major product. The major products are farnesylgeranyl diphosphate and geranylgeranyl diphosphate Micrococcus luteus
A79L mutant enzyme subunit-A(wild-type)/subunit-B(A79L) shows 7fold increased Vmax-values and 6fold decreased Km-values for geranyl diphosphate, which becomes the most preferred substrate of the allylic primers. 3.9fold increase in KM-value for geranylgeranyl diphosphate. Mutation results in shortening the chain length of the major product. The major product is farnesylgeranyl diphosphate Micrococcus luteus
additional information several amino acid residues in the larger subunits Bacillus subtilis heptaprenyl diphosphate synthase are selected for substitutions by site-directed mutagenesis and examined by combination with the corresponding wild type or mutated smaller subunits Micrococcus luteus
V76G mutant enzyme subunit-A(wild-type)/subunit-B(V76G) gives octaprenyl diphosphate as the final products with farnesyl diphosphate as an allylic primer Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) Micrococcus luteus
0.0075
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) Micrococcus luteus
0.0089
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, wild-type enzyme Micrococcus luteus
0.0105
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) Micrococcus luteus
0.0124
-
geranylgeranyl diphosphate pH 8.5, 37°C, wild-type enzyme Micrococcus luteus
0.0137
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) Micrococcus luteus
0.0184
-
isopentenyl diphosphate pH 8.5, 37°C, wild-type enzyme Micrococcus luteus
0.0186
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) Micrococcus luteus
0.0216
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) Micrococcus luteus
0.0216
-
geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) Micrococcus luteus
0.0478
-
geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) Micrococcus luteus
0.385
-
geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus O66127 and O66129 O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase
-
Micrococcus luteus B-P 26 O66127 and O66129 O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate
-
Micrococcus luteus 3 diphosphate + all-trans-hexaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate
-
Micrococcus luteus B-P 26 3 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranyl diphosphate + 4 isopentenyl diphosphate geranyl diphosphate is a poor substrate Micrococcus luteus 4 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranyl diphosphate + 4 isopentenyl diphosphate geranyl diphosphate is a poor substrate Micrococcus luteus B-P 26 4 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
Micrococcus luteus 2 diphosphate + all-trans-hexaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
Micrococcus luteus B-P 26 2 diphosphate + all-trans-hexaprenyl diphosphate
-
?

Synonyms

Synonyms Comment Organism
HexPP synthase
-
Micrococcus luteus