Crystallization (Comment) | Organism |
---|---|
model structure of mutant I112A, calculated using the model of wild-type GFPS as a template. In wild-type enzyme, the I112 on each subunit is located next to the I112 on the other at the dimer interface, to separate the two reaction cavities. In the mutant enzyme, the substitution of I112 by alanine results in the formation of a large tunnel between dimer subunits, enabling prenyl diphosphates to elongate through the newly-created path from one cavity to the other, thus yielding extremely lengthened prenyl diphosphates | Methanosarcina mazei |
Protein Variants | Comment | Organism |
---|---|---|
D109A | mutation leads to the production of longer prenyl diphosphates from C30 to C35, but at small rates | Methanosarcina mazei |
I112A | chain length of the products of mutant I112A reaches up to C70 or longer, which is three times as long as that of the wild type. A large fraction of the longer products is C45-50, which is nearly twice as long as wild-type | Methanosarcina mazei |
II116A | mutation leads to the production of longer prenyl diphosphates from C30 to C35, but at small rates | Methanosarcina mazei |
M137A | slight increase in chain length of products | Methanosarcina mazei |
M72A | slight increase in chain length of products | Methanosarcina mazei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33836 | - |
2 * 33836, calculated | Methanosarcina mazei |
48900 | - |
gel filtration | Methanosarcina mazei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | Q8PYS1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Methanosarcina mazei | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | among mutants, in which the bulky residues that constitute the bottom of the product-accommodating cavity is replaced with alanine, those having mutations on an alpha-helix existing at the subunit interface yield longer products | ? | |
additional information | the residues in chain length determination region III are the predominant factor in the determination of the product specificity | Methanosarcina mazei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33836, calculated | Methanosarcina mazei |
Synonyms | Comment | Organism |
---|---|---|
GFPS | - |
Methanosarcina mazei |