BRENDA - Enzyme Database
show all sequences of 2.5.1.80

A new member of the DMATS superfamily from Aspergillus niger catalyzes prenylations of both tyrosine and tryptophan derivatives

Fan, A.; Chen, H.; Wu, R.; Xu, H.; Li, S.M.; Appl. Microbiol. Biotechnol. 98, 10119-10129 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene An13g01840, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Escherichia coli strain SoluBL21, subcloning in Escherichia coli strain XL1 BlueMRF'
Aspergillus niger
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
strong inhibition
Aspergillus niger
Zn2+
slight inhibition
Aspergillus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
L-tryptophan
pH 7.5, 37°C
Aspergillus niger
0.71
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus niger
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates
Aspergillus niger
Mg2+
activates
Aspergillus niger
additional information
no significant effects on enzyme activity by K+, Na+, Mg2+, Mn2+, Co2+, or EDTA
Aspergillus niger
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52700
-
-
Aspergillus niger
53500
-
-
Aspergillus niger
228000
-
recombinant His6-tagged enzyme, gel filtration
Aspergillus niger
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
A2R1N3
gene An13g01840 or ANI_1_660114
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to near homogeneity by nickel affinity chromatography and desalting gel filtration
Aspergillus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus niger
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-4-methyl-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-5-methoxy-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + L-abrine
-
729072
Aspergillus niger
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + L-beta-homotryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-L-beta-homotryptophan
-
-
-
?
dimethylallyl diphosphate + L-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
-
-
?
additional information
the enzyme catalyzes O-prenylation of L-tyrosine, EC 2.5.1.122, and also accepts 4-amino-L-phenylalanine for an N-prenylation and L-tryptophan for a C7-prenylation, EC 2.5.1.80. HRMS analysis of the enzyme product, overviews. 7-Prenylation substrate specificity, overview. No activity with D-tryptophan
729072
Aspergillus niger
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 53500, recombinant His6-tagged enzyme, SDS-PAGE, x * 52700, about, sequence calculation
Aspergillus niger
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus niger
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0053
-
L-tryptophan
pH 7.5, 37°C
Aspergillus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus niger
Cloned(Commentary) (protein specific)
Commentary
Organism
gene An13g01840, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Escherichia coli strain SoluBL21, subcloning in Escherichia coli strain XL1 BlueMRF'
Aspergillus niger
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
strong inhibition
Aspergillus niger
Zn2+
slight inhibition
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
L-tryptophan
pH 7.5, 37°C
Aspergillus niger
0.71
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus niger
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates
Aspergillus niger
Mg2+
activates
Aspergillus niger
additional information
no significant effects on enzyme activity by K+, Na+, Mg2+, Mn2+, Co2+, or EDTA
Aspergillus niger
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52700
-
-
Aspergillus niger
53500
-
-
Aspergillus niger
228000
-
recombinant His6-tagged enzyme, gel filtration
Aspergillus niger
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to near homogeneity by nickel affinity chromatography and desalting gel filtration
Aspergillus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus niger
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-4-methyl-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-5-methoxy-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan
-
-
-
?
dimethylallyl diphosphate + L-abrine
-
729072
Aspergillus niger
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + L-beta-homotryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-L-beta-homotryptophan
-
-
-
?
dimethylallyl diphosphate + L-tryptophan
-
729072
Aspergillus niger
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
-
-
?
additional information
the enzyme catalyzes O-prenylation of L-tyrosine, EC 2.5.1.122, and also accepts 4-amino-L-phenylalanine for an N-prenylation and L-tryptophan for a C7-prenylation, EC 2.5.1.80. HRMS analysis of the enzyme product, overviews. 7-Prenylation substrate specificity, overview. No activity with D-tryptophan
729072
Aspergillus niger
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53500, recombinant His6-tagged enzyme, SDS-PAGE, x * 52700, about, sequence calculation
Aspergillus niger
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus niger
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0053
-
L-tryptophan
pH 7.5, 37°C
Aspergillus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus niger
General Information
General Information
Commentary
Organism
evolution
the enzyme is a member of the DMATS superfamily
Aspergillus niger
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a member of the DMATS superfamily
Aspergillus niger
Other publictions for EC 2.5.1.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737407
Mai
-
Actions of tryptophan prenyltr ...
Aspergillus fumigatus, Aspergillus fumigatus ATCC MYA-4609
Adv. Synth. Catal.
358
1639 -1653
2016
-
-
1
-
-
-
-
4
-
1
-
2
-
2
-
-
1
-
-
-
-
-
12
-
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
-
2
-
-
-
1
-
-
-
-
12
-
1
-
1
4
1
-
-
-
-
1
1
-
-
-
737406
Winkelblech
-
Tryptophan C5-, C6- and C7-pre ...
Aspergillus fumigatus
Adv. Synth. Catal.
357
975-986
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737544
Fan
Impacts and perspectives of pr ...
Aspergillus fumigatus
Appl. Microbiol. Biotechnol.
99
7399-7415
2015
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
23
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
738647
Fan
Site-directed mutagenesis swit ...
Aspergillus fumigatus
J. Biol. Chem.
290
1364-1373
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739221
Fan
Tryptophan prenyltransferases ...
Aspergillus fumigatus
Org. Biomol. Chem.
13
7551-7557
2015
-
-
-
-
-
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
2
2
-
1
1
729072
Fan
A new member of the DMATS supe ...
Aspergillus niger
Appl. Microbiol. Biotechnol.
98
10119-10129
2014
-
-
1
-
-
-
2
2
-
3
3
-
-
5
-
-
1
-
-
1
-
-
8
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
3
3
-
-
-
-
1
-
1
-
-
8
1
1
-
-
1
1
-
-
-
-
1
1
-
-
-
737837
Miyamoto
A 7-dimethylallyl tryptophan s ...
Neosartorya sp.
Bioorg. Med. Chem.
22
2517-2528
2014
-
-
1
-
-
-
-
6
-
2
2
1
-
5
-
-
1
1
-
-
-
-
10
1
1
-
-
5
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
2
2
1
-
-
-
1
-
-
-
-
10
1
1
-
-
5
1
-
-
-
-
1
1
-
5
5
723104
Yu
Prenyltransferases of the dime ...
Aspergillus clavatus, Aspergillus fischeri
Methods Enzymol.
516
259-278
2012
-
4
2
-
-
-
-
2
-
2
-
2
-
2
-
-
2
-
-
1
-
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
2
-
2
-
2
-
-
-
2
-
1
-
-
6
-
2
-
-
-
2
-
-
-
-
2
2
-
-
-
721373
Yu
Substrate promiscuity of secon ...
Aspergillus fumigatus
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92
737-748
2011
-
2
1
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722044
Yu
Prenylation of flavonoids by u ...
Aspergillus fumigatus
ChemBioChem
12
2280-2283
2011
-
-
1
-
-
-
1
7
-
-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
1
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
7
-
-
-
1
-
-
-
-
-
-
-
-
11
-
1
-
-
7
-
-
-
-
-
1
1
-
7
7
701952
Ruan
Production of diprenylated ind ...
Aspergillus fumigatus
Arch. Microbiol.
191
791-795
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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10
-
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1
1
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-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684649
Kremer
Potential of a 7-dimethylallyl ...
Aspergillus fumigatus
Appl. Microbiol. Biotechnol.
79
951-961
2008
-
-
1
-
-
1
-
-
-
-
3
-
-
2
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-
-
-
-
-
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24
1
-
-
-
-
-
-
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-
1
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1
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3
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24
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
688865
Kremer
A 7-dimethylallyltryptophan sy ...
Aspergillus fumigatus
Microbiology
153
3409-3416
2007
-
-
1
-
-
-
-
2
-
2
1
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5
-
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1
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-
-
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-
5
1
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1
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-
-
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-
2
-
2
1
-
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-
-
1
-
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-
5
1
-
-
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-
-
-
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