Cloned (Comment) | Organism |
---|---|
gene POPACAULIS5 or PttACL5, sequence comparisons and phylogenetic analysis, quantitative real-time RT-PCR expression analysis, functional expression in Saccharomyces cerevisiae cells | Populus tremula x Populus tremuloides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzme overexpression under the control of the 35S CaMV constitutive promoter in Populus tremula x Populus tremuloides plants leads to reduced overall growth and slight defects in xylem development. Ectopic expression of POPACAULIS5 results in the dramatic changes in shoot and root development, and the transgenic lines shows (severe) dwarf phenotypes, overview. Some dwarf transgenic plants are able to develop a rooting system and show partial restoration of the wild-type phenotype once transferred to a plant growth regulator-free medium. Thermospermine accumulation is suppressed in the 35S::POPACAULIS5 Populus woody stem, but not in leaves. Indole-3-acetic acid levels are strongly reduced in the leaves and in the secondary xylem tissues of the transgenic trees | Populus tremula x Populus tremuloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus tremuloides | T1PPE6 | gene POPACAULIS5 or PttACL5 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Populus tremula x Populus tremuloides | - |
stem | - |
Populus tremula x Populus tremuloides | - |
xylem | the enzyme is expressed specifically in early developing xylem vessel elements | Populus tremula x Populus tremuloides | - |
Synonyms | Comment | Organism |
---|---|---|
ACAULIS5 | - |
Populus tremula x Populus tremuloides |
ACL5 | - |
Populus tremula x Populus tremuloides |
POPACAULIS5 | - |
Populus tremula x Populus tremuloides |
Organism | Comment | Expression |
---|---|---|
Populus tremula x Populus tremuloides | exogenous supply of auxin results in a strong increase in POPACAULIS5 transcript levels after 4 h in transgenic overexpressing lines but not in the wild-type. Class III HD-Zip PttHB8 over-expression stimulates POPACAULIS5 expression, mechanism, overview. Expression of PttHB8 is rapidly induced by exogenous auxin and it is therefore possible that the suppression of PttHB8 expression by POPACAULIS5 overexpression is mediated through Indole-3-acetic acid | up |
General Information | Comment | Organism |
---|---|---|
physiological function | thermospermine levels are controlled by an auxin-dependent feedback loop mechanism in Populus xylem. Negative feedback regulation, increased POPACAULIS5 expression functions to reduce indole-3-acetic acid levels, which in turn prevents further expression of POPACAULIS5, a regulatory mechanism, mediated through auxin and PttHB8, for maintenance of thermospermine homeostasis in secondary xylem tissues of the stem | Populus tremula x Populus tremuloides |