Literature summary for 2.5.1.75 extracted from

Khalique, A.; Mattijssen, S.; Haddad, A.F.; Chaudhry, S.; Maraia, R.J.
Targeting mitochondrial and cytosolic substrates of TRIT1 isopentenyltransferase Specificity determinants and tRNA-i6A37 profiles (2020), PLoS Genet., 16, e1008330 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	an N-terminal deletion construct 57-467 is inactive on both the mitochondrial and cytosolic tRNA substrates	Homo sapiens
R17E	mutation in the basic amphipathic helix component of the predicted mitochondrial targeting sequence, mutant accumulates mostly in nuclei and is not found to localize to mitochondria. Mutation decreases modification of mitochondrial but not cytosolic tRNAs upon expression in both Saccharomyces cerevisiae and Schizosaccharomyces pombe	Homo sapiens
R21E	mutation in the basic amphipathic helix component of the predicted mitochondrial targeting sequence, mutant accumulates mostly in nuclei and is not found to localize to mitochondria. Mutation decreases modification of mitochondrial but not cytosolic tRNAs upon expression in both Saccharomyces cerevisiae and Schizosaccharomyces pombe	Homo sapiens
T32A	mutant is inactive on both the mitochondrial and cytosolic tRNA substrates	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-
mitochondrion	Trit1 encodes an amino-terminal mitochondrial targeting sequence that directs mitochondrial import and modification of mitochondrial-tRNAs	Homo sapiens	5739	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9H3H1	-	-

General Information

General Information	Comment	Organism
physiological function	Trit1 exhibits substrate-specific restriction against Schizosaccharomyces pombe cytosolic tRNATrpCCA-A37-A38. Position 32 of tRNATrpCCA may be a conditional determinant for substrate-specific i6A37 modification by Trit1	Homo sapiens

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Release 2023.1 (January 2023)