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Literature summary for 2.5.1.75 extracted from

  • Fradejas, N.; Carlson, B.A.; Rijntjes, E.; Becker, N.P.; Tobe, R.; Schweizer, U.
    Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression (2013), Biochem. J., 450, 427-432.
    View publication on PubMed
Search for more literature for 2.5.1.75

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli, recombinant overexpression of enzyme TRIT1 NIH 3T3 cells, recombinant overexpression of TRIT1 in HepG2 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
D55G site-directed mutagenesis, the mutant shows 50% reduced specific activity compared to the wild-type enzyme Mus musculus
additional information knockdown of enzyme TRIT1 in NIH-3T3 cells by stable expression of shRNA. GPx1 expression is highly sensitive to tRNA[Ser]Sec modification in mouse liver expressing only tRNA[Ser]Sec carrying a 37A>G substitution, GPx1 levels in murine NIH-3T3 cells expressing the Trit1-knockdown construct are significantly reduced compared with control cells Mus musculus
T32A site-directed mutagenesis, the mutant shows 90% reduced specific activity compared to the wild-type enzyme Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec) Mus musculus
-
 diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec)
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q80UN9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant expression of His-tagged enzyme from Escherichia coli by nickel affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
NIH-3T3 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec)
-
 Mus musculus diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec)
-
 ?
dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec) tRNA[Ser]Sec modification assay with [14C]DMAPP and in vitro transcribed tRNA[Ser]Sec. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [14C]dimethylallyl diphosphate to tRNA[Ser]Sec reveals that 37A>G tRNA[Ser]Sec is resistant to isopentenylation by Trit1 Mus musculus diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec)
-
 ?

Synonyms

Synonyms Comment Organism
dimethylallyl:tRNA[Ser]Sec transferase
-
 Mus musculus
DMAPP:tRNA[Ser]Sec transferase
-
 Mus musculus
TRIT1
-
 Mus musculus
tRNA([Ser]Sec)-isopentenyl transferase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

General Information

General Information Comment Organism
malfunction knockdown of Trit1 reduces expression of selenoproteins. Trit1 proteins carrying the D55G or T32A mutations exhibit significantly reduced specific activity of 50% and 10%of wild-type respectively. Overexpression of TRIT1 does not increase selenoprotein expression in HepG2 cells Mus musculus
physiological function mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression, full expression of selenoproteins requires modification of tRNA[Ser]Sec, including N6-isopentenylation of base A37 Mus musculus