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Literature summary for 2.5.1.75 extracted from

  • Soderberg, T.; Poulter, C.D.
    Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: essential elements for recognition of tRNA substrates within the anticodon stem-loop (2000), Biochemistry, 39, 6546-6553.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
GGGGAUUGAGAAUCCCC pH 7.5, 37°C Escherichia coli
0.0033
-
GGGGAUUGAAAGUCCCC pH 7.5, 37°C Escherichia coli
0.0045
-
GGCCAUUGAAAAUGGCC pH 7.5, 37°C Escherichia coli
0.0047
-
GGGGAUUGAAAAUCCCC pH 7.5, 37°C Escherichia coli
0.0065
-
GCGGACUCAAAAUCCGC pH 7.5, 37°C Escherichia coli
0.012
-
GGGCAUUGAAAAUGCCC pH 7.5, 37°C Escherichia coli
0.019
-
GGGGAUUGAAAAAGGGG pH 7.5, 37°C Escherichia coli
0.022
-
GGGAAUUGAAAAUUCCC pH 7.5, 37°C Escherichia coli
0.059
-
GGGGGUUGAAAACCCCC pH 7.5, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + GCGGACUCAAAAUCCGC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GCGGACUCAAAAUCCGC containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGCCAUUGAAAAUGGCC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGCCAUUGAAAAUGGCC containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGGAAUUGAAAAUUCCC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGCCAUUGAAAAUGGCC containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGGCAUUGAAAAUGCCC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGGCAUUGAAAAUGCCC containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGGGAUUGAAAAAGGGG chemically synthesized 17-base RNA random coil oligoribonucleotide based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGGGAUUGAAAAAGGGG containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGGGAUUGAAAAUCCCC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGGGAUUGAAAAAGGGG containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGGGAUUGAAAGUCCCC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGGGAUUGAAAGUCCCC containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGGGAUUGAGAAUCCCC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGGGAUUGAGAAUCCCC containing 6-dimethylallyladenosine
-
?
dimethylallyl diphosphate + GGGGGUUGAAAACCCCC chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe Escherichia coli diphosphate + GGGGGUUGAAAACCCCC containing 6-dimethylallyladenosine
-
?
additional information The A36-A37-A38 motif, which is completely conserved in tRNAs modified by the enzyme, is important for modification. A helix-loop structure is essential for recognition Escherichia coli ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
GGGGAUUGAAAAAGGGG pH 7.5, 37°C Escherichia coli
0.0039
-
GGGGAUUGAAAGUCCCC pH 7.5, 37°C Escherichia coli
0.111
-
GGGGGUUGAAAACCCCC pH 7.5, 37°C Escherichia coli
0.26
-
GCGGACUCAAAAUCCGC pH 7.5, 37°C Escherichia coli
0.67
-
GGGAAUUGAAAAUUCCC pH 7.5, 37°C Escherichia coli
0.67
-
GGCCAUUGAAAAUGGCC pH 7.5, 37°C Escherichia coli
0.75
-
GGGGAUUGAGAAUCCCC pH 7.5, 37°C Escherichia coli
0.79
-
GGGCAUUGAAAAUGCCC pH 7.5, 37°C Escherichia coli
1
-
GGGGAUUGAAAAUCCCC pH 7.5, 37°C Escherichia coli