KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
GGGGAUUGAGAAUCCCC | pH 7.5, 37°C | Escherichia coli | |
0.0033 | - |
GGGGAUUGAAAGUCCCC | pH 7.5, 37°C | Escherichia coli | |
0.0045 | - |
GGCCAUUGAAAAUGGCC | pH 7.5, 37°C | Escherichia coli | |
0.0047 | - |
GGGGAUUGAAAAUCCCC | pH 7.5, 37°C | Escherichia coli | |
0.0065 | - |
GCGGACUCAAAAUCCGC | pH 7.5, 37°C | Escherichia coli | |
0.012 | - |
GGGCAUUGAAAAUGCCC | pH 7.5, 37°C | Escherichia coli | |
0.019 | - |
GGGGAUUGAAAAAGGGG | pH 7.5, 37°C | Escherichia coli | |
0.022 | - |
GGGAAUUGAAAAUUCCC | pH 7.5, 37°C | Escherichia coli | |
0.059 | - |
GGGGGUUGAAAACCCCC | pH 7.5, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + GCGGACUCAAAAUCCGC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GCGGACUCAAAAUCCGC containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGCCAUUGAAAAUGGCC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGCCAUUGAAAAUGGCC containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGGAAUUGAAAAUUCCC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGCCAUUGAAAAUGGCC containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGGCAUUGAAAAUGCCC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGGCAUUGAAAAUGCCC containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGGGAUUGAAAAAGGGG | chemically synthesized 17-base RNA random coil oligoribonucleotide based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGGGAUUGAAAAAGGGG containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGGGAUUGAAAAUCCCC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGGGAUUGAAAAAGGGG containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGGGAUUGAAAGUCCCC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGGGAUUGAAAGUCCCC containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGGGAUUGAGAAUCCCC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGGGAUUGAGAAUCCCC containing 6-dimethylallyladenosine | - |
? | |
dimethylallyl diphosphate + GGGGGUUGAAAACCCCC | chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe | Escherichia coli | diphosphate + GGGGGUUGAAAACCCCC containing 6-dimethylallyladenosine | - |
? | |
additional information | The A36-A37-A38 motif, which is completely conserved in tRNAs modified by the enzyme, is important for modification. A helix-loop structure is essential for recognition | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
GGGGAUUGAAAAAGGGG | pH 7.5, 37°C | Escherichia coli | |
0.0039 | - |
GGGGAUUGAAAGUCCCC | pH 7.5, 37°C | Escherichia coli | |
0.111 | - |
GGGGGUUGAAAACCCCC | pH 7.5, 37°C | Escherichia coli | |
0.26 | - |
GCGGACUCAAAAUCCGC | pH 7.5, 37°C | Escherichia coli | |
0.67 | - |
GGGAAUUGAAAAUUCCC | pH 7.5, 37°C | Escherichia coli | |
0.67 | - |
GGCCAUUGAAAAUGGCC | pH 7.5, 37°C | Escherichia coli | |
0.75 | - |
GGGGAUUGAGAAUCCCC | pH 7.5, 37°C | Escherichia coli | |
0.79 | - |
GGGCAUUGAAAAUGCCC | pH 7.5, 37°C | Escherichia coli | |
1 | - |
GGGGAUUGAAAAUCCCC | pH 7.5, 37°C | Escherichia coli |