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Literature summary for 2.5.1.75 extracted from
- Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: a binding mechanism for recombinant enzyme (1997), Biochemistry, 36, 604-614.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000096 | - |
adenosine 37 in tRNA-Phe | pH 7.5, 37°C | Escherichia coli | |
| 0.0032 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34700 | - |
1 * 34700, SDS-PAGE | Escherichia coli |
| 37800 | - |
gel fitlration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dimethylallyl diphosphate + adenine37 in tRNA = diphosphate + N6-dimethylallyladenine37 in tRNA | ordered sequential mechanism for substrate binding | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.15 | - |
pH 7.5, 37°C | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -78°C, stable for several months | Escherichia coli |
| 0°C, stable for at least one week | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + adenine37 in tRNAPhe | - |
Escherichia coli | diphosphate + N6-dimethylallyladenine37 in tRNAPhe | enzyme transfers the dimethylallyl moiety of dimethylallyl diphosphate to A37, located adjacent to the anticodon in undermodified tRNAPhe | ? | |
| dimethylallyl diphosphate + adenosine 37 in tRNA-Phe | - |
Escherichia coli | N6-(dimethylallyl)adenosine37 in tRNAPhe + diphosphate | - |
? | |
| additional information | enzyme does not bind dimethylallyl diphosphate in absence of tRNA | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34700, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | broad | Escherichia coli |
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