Literature summary for 2.5.1.70 extracted from

Yamamoto, H.; Senda, M.; Inoue, K.
Flavanone 8-dimethylallyltransferase in Sophora flavescens cell suspension cultures (2000), Phytochemistry, 54, 649-655.

Search for more literature for 2.5.1.70

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.036	-	(2S)-naringenin	pH 9.0, 25°C, microsome	Sophora flavescens
0.12	-	3,3-dimethylallyl diphosphate	pH 9.0, 25°C, microsome	Sophora flavescens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Sophora flavescens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	6% of the activity with Mg2+	Sophora flavescens
Co2+	5% of the activity with Mg2+	Sophora flavescens
Cu2+	1% of the activity with Mg2+	Sophora flavescens
Mg2+	required, best divalent cation, optimal at 10 mM	Sophora flavescens
Mn2+	32% of the activity with Mg2+	Sophora flavescens
Zn2+	2% of the activity with Mg2+	Sophora flavescens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3,3-dimethylallyl diphosphate + (2S)-naringenin	Sophora flavescens	two possible pathways leading to sophoraflavanone G, overview	diphosphate + sophoraflavanone B	-	?

Organism

Organism	UniProt	Comment	Textmining
Sophora flavescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme partially by microsome preparation	Sophora flavescens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,3-dimethylallyl diphosphate + (2S)-liquiritigenin	i.e. 4',7-dihydroxyflavanone	Sophora flavescens	diphosphate + isobavachin	-	?
3,3-dimethylallyl diphosphate + (2S)-naringenin	two possible pathways leading to sophoraflavanone G, overview	Sophora flavescens	diphosphate + sophoraflavanone B	-	?
3,3-dimethylallyl diphosphate + (2S)-naringenin	best substrate, the enzyme is enantiospecific for (2S)-naringenin, i.e. (S)-4',5,7-trihydroxy-flavanone, and utilizes 3,3-dimethylallyl diphosphate as sole prenyl donor	Sophora flavescens	diphosphate + sophoraflavanone B	-	?
3,3-dimethylallyl diphosphate + 2'-hydroxynaringenin	with 2'-hydroxynaringenin, i.e. (S)-2',4',5,7-tetrahydroxy-flavanone, the enzyme shows 3% of the activity with naringenin	Sophora flavescens	diphosphate + leachianone G	-	?
3,3-dimethylallyl diphosphate + hesperetin	i.e. 5,7,3'-trihydroxy-4'-methoxyflavanone	Sophora flavescens	diphosphate + (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-8-(3-methylbut-2-en-1-yl)-2,3-dihydro-4H-chromen-4-one	-	?
3,3-dimethylallyl diphosphate + taxifolin	i.e. 3,5,7,3',4'-pentahydroxyflavanone	Sophora flavescens	diphosphate + (2R,3R)-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-8-(3-methylbut-2-en-1-yl)-2,3-dihydro-4H-chromen-4-one	-	?
additional information	substrate specificity, formation of 8-dimethylallyl-, 8-lavandulyl- and 6-dimethylallylflavanones, overview	Sophora flavescens	?	-	?

Synonyms

Synonyms	Comment	Organism
flavanone 8-dimethylallyltransferase	-	Sophora flavescens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Sophora flavescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	10	-	Sophora flavescens

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.5	10	-	Sophora flavescens

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Release 2023.1 (January 2023)