Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.7 extracted from

  • Sonkar, A.; Shukla, H.; Shukla, R.; Kalita, J.; Tripathi, T.
    Unfolding of Acinetobacter baumannii MurA proceeds through a metastable intermediate A combined spectroscopic and computational investigation (2019), Int. J. Biol. Macromol., 126, 941-951 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine Acinetobacter baumannii the enzyme catalyzes the first committed step of peptidoglycan biosynthesis phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
urea the urea-induced unfolding of the enzyme is a three-state process, where a metastable intermediate conformation state is populated between 3.0-4.0 M. Above 6.0 M urea, the enzyme gets completely unfolded. The transition from the native structure to the partially unfolded metastable state involves about 30% loss of native contacts but little change in the radius of gyration or core hydration properties. The intermediate-to-unfolded state transition is characterized by a large increase in the radius of gyration. Molecular dynamics trajectories simulated in different unfolding conditions suggest that urea destabilizes the enzyme structure weakening hydrophobic interactions and the hydrogen bond network Acinetobacter baumannii

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine
-
Acinetobacter baumannii phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
?
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine the enzyme catalyzes the first committed step of peptidoglycan biosynthesis Acinetobacter baumannii phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
MurA
-
Acinetobacter baumannii

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first committed step of peptidoglycan biosynthesis Acinetobacter baumannii