Literature summary for 2.5.1.7 extracted from

Zhu, J.Y.; Yang, Y.; Han, H.; Betzi, S.; Olesen, S.H.; Marsilio, F.; Schoenbrunn, E.
Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA) (2012), J. Biol. Chem., 287, 12657-12667.

Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Enterobacter cloacae

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with substrates, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 5.5), 0.2 M NH4OAc, 25% (w/v) PEG 3350	Enterobacter cloacae

Protein Variants

Protein Variants	Comment	Organism
C115D	the mutant enzyme lacks the ability to react with phosphoenolpyruvate covalently	Enterobacter cloacae

Inhibitors

Inhibitors	Comment	Organism	Structure
fosfomycin	-	Enterobacter cloacae

Organism

Organism	UniProt	Comment	Textmining
Enterobacter cloacae	P33038	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Q-Sepharose column chromatography, Superdex 200 gel filtration, and Sephadex G-25 gel filtration	Enterobacter cloacae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme does not display appreciable catalytic activity with UDP-N-acetyl-D-galactosamine as a substrate	Enterobacter cloacae	?	-	?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine	-	Enterobacter cloacae	UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate	-	r

Synonyms

Synonyms	Comment	Organism
MurA	-	Enterobacter cloacae
UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-	Enterobacter cloacae

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Release 2023.1 (January 2023)