Cloned (Comment) | Organism |
---|---|
expressed in an Enterococcus faecalis mutant lacking IreK (formerly PrkC), a key kinase required for cephalosporin resistance, and in Escherichia coli BL21(DE3) cells | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
C120S | catalytically inactive | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterococcus faecalis | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurAA | - |
Enterococcus faecalis |
UDP-N-acetylglucosamine 1-carboxyvinyl transferase | - |
Enterococcus faecalis |
General Information | Comment | Organism |
---|---|---|
malfunction | frame deletion of murAA leads to increased susceptibility of Enterococcus faecalis to the cephalosporins ceftriaxone and ceftazidime | Enterococcus faecalis |
physiological function | the enzyme is necessary but not sufficient for intrinsic cephalosporin resistance of Enterococcus faecalis | Enterococcus faecalis |