Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Aliivibrio fischeri |
Crystallization (Comment) | Organism |
---|---|
in complex with UDP-N-acetylglucosamine and fosfomycin, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M bis-Tris pH 6.5 | Aliivibrio fischeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | - |
Aliivibrio fischeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45640 | - |
x * 45640, calculated from amino acid sequence | Aliivibrio fischeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | B5F9P4 | - |
- |
Aliivibrio fischeri MJ11 | B5F9P4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 75 gel filtration | Aliivibrio fischeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Aliivibrio fischeri | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Aliivibrio fischeri MJ11 | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45640, calculated from amino acid sequence | Aliivibrio fischeri |
Synonyms | Comment | Organism |
---|---|---|
MurA | - |
Aliivibrio fischeri |
UDP-N-acetylglucosamine enolpyruvyl transferase | - |
Aliivibrio fischeri |